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Isolation of the domain containing the molybdenum, iron-sulfur I, and iron-sulfur II centers of chicken liver xanthine dehydrogenase.

Publication ,  Journal Article
Coughlan, MP; Betcher-Lange, SL; Rajagopalan, KV
Published in: J Biol Chem
November 10, 1979

Chicken liver xanthine dehydrogenase, like other xanthine-oxidizing enzymes, is a dimer of Mr = 150,000 subunits. Each subunit contains one molybdenum, one FAD, and two distinct Fe2S2 centers. Treatment with a number of proteases shows that the native enzyme subunit is cleaved at three distinct sites. However, the cleavage products can be separated only under denaturing conditions. Prolonged treatment with subtilisin at pH 10.1 has permitted the isolation of an Mr = 65,000 catalytically active fragment that is devoid of FAD but which contains the molybdenum and both types of iron-sulfur center. A model of the domain structure of the native enzyme is proposed.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 10, 1979

Volume

254

Issue

21

Start / End Page

10694 / 10699

Location

United States

Related Subject Headings

  • Xanthine Dehydrogenase
  • Spectrophotometry
  • Peptide Hydrolases
  • Molybdenum
  • Molecular Weight
  • Metalloproteins
  • Macromolecular Substances
  • Liver
  • Ketone Oxidoreductases
  • Iron-Sulfur Proteins
 

Citation

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Coughlan, M. P., Betcher-Lange, S. L., & Rajagopalan, K. V. (1979). Isolation of the domain containing the molybdenum, iron-sulfur I, and iron-sulfur II centers of chicken liver xanthine dehydrogenase. J Biol Chem, 254(21), 10694–10699.
Coughlan, M. P., S. L. Betcher-Lange, and K. V. Rajagopalan. “Isolation of the domain containing the molybdenum, iron-sulfur I, and iron-sulfur II centers of chicken liver xanthine dehydrogenase.J Biol Chem 254, no. 21 (November 10, 1979): 10694–99.
Coughlan MP, Betcher-Lange SL, Rajagopalan KV. Isolation of the domain containing the molybdenum, iron-sulfur I, and iron-sulfur II centers of chicken liver xanthine dehydrogenase. J Biol Chem. 1979 Nov 10;254(21):10694–9.
Coughlan MP, Betcher-Lange SL, Rajagopalan KV. Isolation of the domain containing the molybdenum, iron-sulfur I, and iron-sulfur II centers of chicken liver xanthine dehydrogenase. J Biol Chem. 1979 Nov 10;254(21):10694–10699.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 10, 1979

Volume

254

Issue

21

Start / End Page

10694 / 10699

Location

United States

Related Subject Headings

  • Xanthine Dehydrogenase
  • Spectrophotometry
  • Peptide Hydrolases
  • Molybdenum
  • Molecular Weight
  • Metalloproteins
  • Macromolecular Substances
  • Liver
  • Ketone Oxidoreductases
  • Iron-Sulfur Proteins