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In vitro reconstitution of nitrate reductase activity of the Neurospora crassa mutant nit-1: specific incorporation of molybdopterin.

Publication ,  Journal Article
Kramer, S; Hageman, RV; Rajagopalan, KV
Published in: Arch Biochem Biophys
September 1984

The reduced, metal-free pterin of the molybdenum cofactor has been termed molybdopterin. Oxidation of any molybdopterin-containing protein in the presence or absence of iodine yields oxidized molybdopterin derivatives termed Form A and Form B, respectively. Application of these procedures to whole cells and cell extracts has demonstrated the presence of molybdopterin in wild-type Neurospora crassa, and its absence in the cofactor-deficient mutant nit-1. In order to demonstrate that the reconstitution of nitrate reductase activity in nit-1 extracts results from the incorporation of molybdopterin into the apoprotein, active molybdopterin, free of contaminating amino acids or peptides, was isolated from chicken liver sulfite oxidase and used in the reconstitution system. The results show that, during reconstitution, exogenous molybdopterin is specifically incorporated into the nitrate reductase protein, confirming the role of molybdopterin as the organic moiety of the molybdenum cofactor.

Duke Scholars

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

September 1984

Volume

233

Issue

2

Start / End Page

821 / 829

Location

United States

Related Subject Headings

  • Spectrometry, Fluorescence
  • Pteridines
  • Nitrate Reductases
  • Neurospora crassa
  • Neurospora
  • Mutation
  • Molybdenum Cofactors
  • Molybdenum
  • Metalloproteins
  • Coenzymes
 

Citation

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Kramer, S., Hageman, R. V., & Rajagopalan, K. V. (1984). In vitro reconstitution of nitrate reductase activity of the Neurospora crassa mutant nit-1: specific incorporation of molybdopterin. Arch Biochem Biophys, 233(2), 821–829. https://doi.org/10.1016/0003-9861(84)90511-3
Kramer, S., R. V. Hageman, and K. V. Rajagopalan. “In vitro reconstitution of nitrate reductase activity of the Neurospora crassa mutant nit-1: specific incorporation of molybdopterin.Arch Biochem Biophys 233, no. 2 (September 1984): 821–29. https://doi.org/10.1016/0003-9861(84)90511-3.
Kramer, S., et al. “In vitro reconstitution of nitrate reductase activity of the Neurospora crassa mutant nit-1: specific incorporation of molybdopterin.Arch Biochem Biophys, vol. 233, no. 2, Sept. 1984, pp. 821–29. Pubmed, doi:10.1016/0003-9861(84)90511-3.
Journal cover image

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

September 1984

Volume

233

Issue

2

Start / End Page

821 / 829

Location

United States

Related Subject Headings

  • Spectrometry, Fluorescence
  • Pteridines
  • Nitrate Reductases
  • Neurospora crassa
  • Neurospora
  • Mutation
  • Molybdenum Cofactors
  • Molybdenum
  • Metalloproteins
  • Coenzymes