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Rat liver L-glutamate dehydrogenase, malate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, and sulfite oxidase are each synthesized as larger precursors by cytoplasmic free polysomes.

Publication ,  Journal Article
Mihara, K; Omura, T; Harano, T; Brenner, S; Fleischer, S; Rajagopalan, KV; Blobel, G
Published in: J Biol Chem
April 10, 1982

Four cytoplasmically synthesized rat liver mitochondrial enzymes, located either as soluble enzymes in the mitochondrial matrix (L-glutamate dehydrogenase and malate dehydrogenase or in the intermembrane space (sulfite oxidase) or as an integral membrane protein located on the matrix face of the inner mitochondrial membrane (D-beta-hydroxybutyrate dehydrogenase), were all shown to be synthesized as precursors larger than their mature counterparts by 1000-6000 daltons. These larger forms were detected in vitro, in a cell-free protein synthesizing system programmed with either total rat liver RNA or with RNA isolated from free polysomes or with free polysomes, and in vivo, in the two cases that were investigated (L-glutamate dehydrogenase and D-beta-hydroxybutyrate dehydrogenase), by pulse labeling of Buffalo rat liver cells in culture. The intracellular site of synthesis of all four mitochondrial enzymes was shown to be primarily on free polysomes and not on membrane-bound polysomes.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 10, 1982

Volume

257

Issue

7

Start / End Page

3355 / 3358

Location

United States

Related Subject Headings

  • Rats
  • RNA, Ribosomal
  • Protein Biosynthesis
  • Polyribosomes
  • Oxidoreductases Acting on Sulfur Group Donors
  • Oxidoreductases
  • Molecular Weight
  • Mitochondria, Liver
  • Malate Dehydrogenase
  • Liver
 

Citation

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Mihara, K., Omura, T., Harano, T., Brenner, S., Fleischer, S., Rajagopalan, K. V., & Blobel, G. (1982). Rat liver L-glutamate dehydrogenase, malate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, and sulfite oxidase are each synthesized as larger precursors by cytoplasmic free polysomes. J Biol Chem, 257(7), 3355–3358.
Mihara, K., T. Omura, T. Harano, S. Brenner, S. Fleischer, K. V. Rajagopalan, and G. Blobel. “Rat liver L-glutamate dehydrogenase, malate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, and sulfite oxidase are each synthesized as larger precursors by cytoplasmic free polysomes.J Biol Chem 257, no. 7 (April 10, 1982): 3355–58.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 10, 1982

Volume

257

Issue

7

Start / End Page

3355 / 3358

Location

United States

Related Subject Headings

  • Rats
  • RNA, Ribosomal
  • Protein Biosynthesis
  • Polyribosomes
  • Oxidoreductases Acting on Sulfur Group Donors
  • Oxidoreductases
  • Molecular Weight
  • Mitochondria, Liver
  • Malate Dehydrogenase
  • Liver