
Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum.
Publication
, Journal Article
Johnson, JL; Bastian, NR; Schauer, NL; Ferry, JG; Rajagopalan, KV
Published in: FEMS Microbiol Lett
January 15, 1991
The pterin cofactor in formate dehydrogenase isolated from Methanobacterium formicium is identified as molybdopterin guanine dinucleotide. The pterin, stabilized as the alkylated, dicarboxamidomethyl derivative, is shown to have absorption and chromatographic properties identical to those of the previously characterized authentic compound. Treatment with nucleotide pyrophosphatase produced the expected degradation products GMP and carboxyamidomethyl molybdopterin. The molybdopterin guanine dinucleotide released from the enzyme by treatment with 95% dimethyl sulfoxide is shown to be functional in the in vitro reconstitution of the cofactor-deficient nitrate reductase in extracts of the Neurospora crassa nit-1 mutant.
Duke Scholars
Published In
FEMS Microbiol Lett
DOI
ISSN
0378-1097
Publication Date
January 15, 1991
Volume
61
Issue
2-3
Start / End Page
213 / 216
Location
England
Related Subject Headings
- Spectrophotometry
- Pterins
- Microbiology
- Guanine Nucleotides
- Formate Dehydrogenases
- Euryarchaeota
- Chromatography, High Pressure Liquid
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 30 Agricultural, veterinary and food sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Johnson, J. L., Bastian, N. R., Schauer, N. L., Ferry, J. G., & Rajagopalan, K. V. (1991). Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum. FEMS Microbiol Lett, 61(2–3), 213–216. https://doi.org/10.1016/0378-1097(91)90554-n
Johnson, J. L., N. R. Bastian, N. L. Schauer, J. G. Ferry, and K. V. Rajagopalan. “Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum.” FEMS Microbiol Lett 61, no. 2–3 (January 15, 1991): 213–16. https://doi.org/10.1016/0378-1097(91)90554-n.
Johnson JL, Bastian NR, Schauer NL, Ferry JG, Rajagopalan KV. Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum. FEMS Microbiol Lett. 1991 Jan 15;61(2–3):213–6.
Johnson, J. L., et al. “Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum.” FEMS Microbiol Lett, vol. 61, no. 2–3, Jan. 1991, pp. 213–16. Pubmed, doi:10.1016/0378-1097(91)90554-n.
Johnson JL, Bastian NR, Schauer NL, Ferry JG, Rajagopalan KV. Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum. FEMS Microbiol Lett. 1991 Jan 15;61(2–3):213–216.

Published In
FEMS Microbiol Lett
DOI
ISSN
0378-1097
Publication Date
January 15, 1991
Volume
61
Issue
2-3
Start / End Page
213 / 216
Location
England
Related Subject Headings
- Spectrophotometry
- Pterins
- Microbiology
- Guanine Nucleotides
- Formate Dehydrogenases
- Euryarchaeota
- Chromatography, High Pressure Liquid
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 30 Agricultural, veterinary and food sciences