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Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum.

Publication ,  Journal Article
Johnson, JL; Bastian, NR; Schauer, NL; Ferry, JG; Rajagopalan, KV
Published in: FEMS Microbiol Lett
January 15, 1991

The pterin cofactor in formate dehydrogenase isolated from Methanobacterium formicium is identified as molybdopterin guanine dinucleotide. The pterin, stabilized as the alkylated, dicarboxamidomethyl derivative, is shown to have absorption and chromatographic properties identical to those of the previously characterized authentic compound. Treatment with nucleotide pyrophosphatase produced the expected degradation products GMP and carboxyamidomethyl molybdopterin. The molybdopterin guanine dinucleotide released from the enzyme by treatment with 95% dimethyl sulfoxide is shown to be functional in the in vitro reconstitution of the cofactor-deficient nitrate reductase in extracts of the Neurospora crassa nit-1 mutant.

Duke Scholars

Published In

FEMS Microbiol Lett

DOI

ISSN

0378-1097

Publication Date

January 15, 1991

Volume

61

Issue

2-3

Start / End Page

213 / 216

Location

England

Related Subject Headings

  • Spectrophotometry
  • Pterins
  • Microbiology
  • Guanine Nucleotides
  • Formate Dehydrogenases
  • Euryarchaeota
  • Chromatography, High Pressure Liquid
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences
 

Citation

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Johnson, J. L., Bastian, N. R., Schauer, N. L., Ferry, J. G., & Rajagopalan, K. V. (1991). Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum. FEMS Microbiol Lett, 61(2–3), 213–216. https://doi.org/10.1016/0378-1097(91)90554-n
Johnson, J. L., N. R. Bastian, N. L. Schauer, J. G. Ferry, and K. V. Rajagopalan. “Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum.FEMS Microbiol Lett 61, no. 2–3 (January 15, 1991): 213–16. https://doi.org/10.1016/0378-1097(91)90554-n.
Johnson JL, Bastian NR, Schauer NL, Ferry JG, Rajagopalan KV. Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum. FEMS Microbiol Lett. 1991 Jan 15;61(2–3):213–6.
Johnson, J. L., et al. “Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum.FEMS Microbiol Lett, vol. 61, no. 2–3, Jan. 1991, pp. 213–16. Pubmed, doi:10.1016/0378-1097(91)90554-n.
Johnson JL, Bastian NR, Schauer NL, Ferry JG, Rajagopalan KV. Identification of molybdopterin guanine dinucleotide in formate dehydrogenase from Methanobacterium formicicum. FEMS Microbiol Lett. 1991 Jan 15;61(2–3):213–216.
Journal cover image

Published In

FEMS Microbiol Lett

DOI

ISSN

0378-1097

Publication Date

January 15, 1991

Volume

61

Issue

2-3

Start / End Page

213 / 216

Location

England

Related Subject Headings

  • Spectrophotometry
  • Pterins
  • Microbiology
  • Guanine Nucleotides
  • Formate Dehydrogenases
  • Euryarchaeota
  • Chromatography, High Pressure Liquid
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences