The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants.
Publication
, Journal Article
Hamm-Alvarez, SF; Sancar, A; Rajagopalan, KV
Published in: J Biol Chem
June 15, 1990
Escherichia coli DNA photolyase was overproduced and purified from each of two mutant E. coli strains lacking dihydrofolate reductase. The extent of over-production in the mutants was comparable to that seen in the wild type strain. Examination of the isolated photolyase from these strains revealed that the folate cofactor, 5,10-methenyltetrahydrofolate, was present in these proteins at a level of 60-80% compared to that purified from the wild type strain. Further examination of the dihydrofolate reductase-deficient strains revealed the presence of other tetrahydrofolate derivatives. These findings demonstrate that dihydrofolate reductase is not essential for the production of tetrahydrofolates in E. coli.
Duke Scholars
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
June 15, 1990
Volume
265
Issue
17
Start / End Page
9850 / 9856
Location
United States
Related Subject Headings
- Tetrahydrofolate Dehydrogenase
- Mutation
- Genotype
- Folic Acid
- Escherichia coli
- Deoxyribodipyrimidine Photo-Lyase
- Chromatography, High Pressure Liquid
- Biochemistry & Molecular Biology
- 34 Chemical sciences
- 32 Biomedical and clinical sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Hamm-Alvarez, S. F., Sancar, A., & Rajagopalan, K. V. (1990). The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants. J Biol Chem, 265(17), 9850–9856.
Hamm-Alvarez, S. F., A. Sancar, and K. V. Rajagopalan. “The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants.” J Biol Chem 265, no. 17 (June 15, 1990): 9850–56.
Hamm-Alvarez SF, Sancar A, Rajagopalan KV. The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants. J Biol Chem. 1990 Jun 15;265(17):9850–6.
Hamm-Alvarez, S. F., et al. “The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants.” J Biol Chem, vol. 265, no. 17, June 1990, pp. 9850–56.
Hamm-Alvarez SF, Sancar A, Rajagopalan KV. The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants. J Biol Chem. 1990 Jun 15;265(17):9850–9856.
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
June 15, 1990
Volume
265
Issue
17
Start / End Page
9850 / 9856
Location
United States
Related Subject Headings
- Tetrahydrofolate Dehydrogenase
- Mutation
- Genotype
- Folic Acid
- Escherichia coli
- Deoxyribodipyrimidine Photo-Lyase
- Chromatography, High Pressure Liquid
- Biochemistry & Molecular Biology
- 34 Chemical sciences
- 32 Biomedical and clinical sciences