Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides.
Publication
, Journal Article
Hilton, JC; Rajagopalan, KV
Published in: Biochim Biophys Acta
May 23, 1996
The dsrA gene encoding the molybdoenzyme dimethyl sulfoxide reductase was isolated by screening phagemid libraries containing restriction fragments of Rhodobacter sphaeroides f. sp. denitrificans genomic DNA with a pool of degenerate oligonucleotides. The encoded 822 amino-acid protein includes a 42 amino-acid periplasmic signal sequence that is cleaved during activation of the enzyme. Both forms of the protein were heterologously expressed in inactive states in E. coli and identified by Western blot analysis.
Duke Scholars
Published In
Biochim Biophys Acta
DOI
ISSN
0006-3002
Publication Date
May 23, 1996
Volume
1294
Issue
2
Start / End Page
111 / 114
Location
Netherlands
Related Subject Headings
- Rhodobacter sphaeroides
- Recombinant Proteins
- Polymerase Chain Reaction
- Oxidoreductases
- Mutagenesis, Site-Directed
- Molecular Sequence Data
- Iron-Sulfur Proteins
- Genetic Vectors
- Genes, Bacterial
- DNA Primers
Citation
APA
Chicago
ICMJE
MLA
NLM
Hilton, J. C., & Rajagopalan, K. V. (1996). Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides. Biochim Biophys Acta, 1294(2), 111–114. https://doi.org/10.1016/0167-4838(96)00015-5
Hilton, J. C., and K. V. Rajagopalan. “Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides.” Biochim Biophys Acta 1294, no. 2 (May 23, 1996): 111–14. https://doi.org/10.1016/0167-4838(96)00015-5.
Hilton JC, Rajagopalan KV. Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides. Biochim Biophys Acta. 1996 May 23;1294(2):111–4.
Hilton, J. C., and K. V. Rajagopalan. “Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides.” Biochim Biophys Acta, vol. 1294, no. 2, May 1996, pp. 111–14. Pubmed, doi:10.1016/0167-4838(96)00015-5.
Hilton JC, Rajagopalan KV. Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides. Biochim Biophys Acta. 1996 May 23;1294(2):111–114.
Published In
Biochim Biophys Acta
DOI
ISSN
0006-3002
Publication Date
May 23, 1996
Volume
1294
Issue
2
Start / End Page
111 / 114
Location
Netherlands
Related Subject Headings
- Rhodobacter sphaeroides
- Recombinant Proteins
- Polymerase Chain Reaction
- Oxidoreductases
- Mutagenesis, Site-Directed
- Molecular Sequence Data
- Iron-Sulfur Proteins
- Genetic Vectors
- Genes, Bacterial
- DNA Primers