Selenite binding to carbon monoxide oxidase from Pseudomonas carboxydovorans. Selenium binds covalently to the protein and activates specifically the CO----methylene blue reaction.
Publication
, Journal Article
Meyer, O; Rajagopalan, KV
Published in: J Biol Chem
May 10, 1984
The CO----methylene blue and CO----dichlorophenol indophenol activities of carbon monoxide oxidase were specifically activated upon aerobic incubation with selenite, whereas the NADH----methylene blue activity was not altered. Fully active enzyme contained selenium, molybdenum, and flavin adenine dinucleotide in a 1:1:1 ratio. Selenium was covalently bound to the protein, probably between the sulfurs of half-cystine residues, and not a constituent of the molybdenum cofactor. The action of selenite was directed to the cytoplasmic species of carbon monoxide oxidase exclusively, whereas the CO----methylene blue activity of the membrane-bound enzyme remained unaffected.
Duke Scholars
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
May 10, 1984
Volume
259
Issue
9
Start / End Page
5612 / 5617
Location
United States
Related Subject Headings
- Selenium
- Selenious Acid
- Pseudomonas
- Protein Binding
- Methylene Blue
- Kinetics
- Enzyme Activation
- Biochemistry & Molecular Biology
- Aldehyde Oxidoreductases
- 34 Chemical sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Meyer, O., & Rajagopalan, K. V. (1984). Selenite binding to carbon monoxide oxidase from Pseudomonas carboxydovorans. Selenium binds covalently to the protein and activates specifically the CO----methylene blue reaction. J Biol Chem, 259(9), 5612–5617.
Meyer, O., and K. V. Rajagopalan. “Selenite binding to carbon monoxide oxidase from Pseudomonas carboxydovorans. Selenium binds covalently to the protein and activates specifically the CO----methylene blue reaction.” J Biol Chem 259, no. 9 (May 10, 1984): 5612–17.
Meyer O, Rajagopalan KV. Selenite binding to carbon monoxide oxidase from Pseudomonas carboxydovorans. Selenium binds covalently to the protein and activates specifically the CO----methylene blue reaction. J Biol Chem. 1984 May 10;259(9):5612–7.
Meyer, O., and K. V. Rajagopalan. “Selenite binding to carbon monoxide oxidase from Pseudomonas carboxydovorans. Selenium binds covalently to the protein and activates specifically the CO----methylene blue reaction.” J Biol Chem, vol. 259, no. 9, May 1984, pp. 5612–17.
Meyer O, Rajagopalan KV. Selenite binding to carbon monoxide oxidase from Pseudomonas carboxydovorans. Selenium binds covalently to the protein and activates specifically the CO----methylene blue reaction. J Biol Chem. 1984 May 10;259(9):5612–5617.
Published In
J Biol Chem
ISSN
0021-9258
Publication Date
May 10, 1984
Volume
259
Issue
9
Start / End Page
5612 / 5617
Location
United States
Related Subject Headings
- Selenium
- Selenious Acid
- Pseudomonas
- Protein Binding
- Methylene Blue
- Kinetics
- Enzyme Activation
- Biochemistry & Molecular Biology
- Aldehyde Oxidoreductases
- 34 Chemical sciences