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31P-NMR of free and protein-bound molybdopterin guanine dinucleotide.

Publication ,  Journal Article
Bastian, NR; Johnson, JL; Rajagopalan, KV
Published in: Biofactors
January 1992

Molybdopterin guanine dinucleotide was studied by 31P-NMR in the free, iodoacetamide derivatized form [di(carboxamidomethyl)molybdopterin] and in the native state in the dimethyl sulfoxide reductase from Rhodobacter sphaeroides. The spectra confirm the presence of a pyrophosphate moiety in the cofactor molecule. Comparison of the spectrum of the free pterin with that of the protein-bound cofactor reveals a substantial upfield shift of the 31P resonances in the enzyme-bound form with respect to the free form. This shift is attributed to differences in the bond and torsional angles of the phosphates. The spectrum of the protein suggests significant coupling between the two phosphorus nuclei with coupling constants of approximately 200 Hz. Comparison of the 31P-NMR spectra of molybdopterin guanine dinucleotide and flavin adenine dinucleotide suggests that the two cofactors have similar conformations in both their free and protein-bound forms.

Duke Scholars

Published In

Biofactors

ISSN

0951-6433

Publication Date

January 1992

Volume

3

Issue

3

Start / End Page

197 / 200

Location

Netherlands

Related Subject Headings

  • Rhodobacter sphaeroides
  • Pterins
  • Protein Binding
  • Phosphorus Radioisotopes
  • Magnetic Resonance Spectroscopy
  • Guanine Nucleotides
  • Cells, Cultured
  • Biochemistry & Molecular Biology
  • Animals
  • 3101 Biochemistry and cell biology
 

Citation

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Bastian, N. R., Johnson, J. L., & Rajagopalan, K. V. (1992). 31P-NMR of free and protein-bound molybdopterin guanine dinucleotide. Biofactors, 3(3), 197–200.
Bastian, N. R., J. L. Johnson, and K. V. Rajagopalan. “31P-NMR of free and protein-bound molybdopterin guanine dinucleotide.Biofactors 3, no. 3 (January 1992): 197–200.
Bastian NR, Johnson JL, Rajagopalan KV. 31P-NMR of free and protein-bound molybdopterin guanine dinucleotide. Biofactors. 1992 Jan;3(3):197–200.
Bastian, N. R., et al. “31P-NMR of free and protein-bound molybdopterin guanine dinucleotide.Biofactors, vol. 3, no. 3, Jan. 1992, pp. 197–200.
Bastian NR, Johnson JL, Rajagopalan KV. 31P-NMR of free and protein-bound molybdopterin guanine dinucleotide. Biofactors. 1992 Jan;3(3):197–200.
Journal cover image

Published In

Biofactors

ISSN

0951-6433

Publication Date

January 1992

Volume

3

Issue

3

Start / End Page

197 / 200

Location

Netherlands

Related Subject Headings

  • Rhodobacter sphaeroides
  • Pterins
  • Protein Binding
  • Phosphorus Radioisotopes
  • Magnetic Resonance Spectroscopy
  • Guanine Nucleotides
  • Cells, Cultured
  • Biochemistry & Molecular Biology
  • Animals
  • 3101 Biochemistry and cell biology