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The kinetic mechanism of xanthine dehydrogenase and related enzymes.

Publication ,  Journal Article
Coughlan, MP; Rajagopalan, KV
Published in: Eur J Biochem
March 1980

Xanthine dehydrogenase and related enzymes contain multicomponent internal electron transfer chains. The topographical arrangement of this chain in such that the oxidation of substrate and the reduction of the electron acceptor occur at separate non-overlapping sites that communicate by intramolecular electron transfer. The steady-state kinetic behaviour of these enzymes is thus ideally suited to a two-site ping-pong mechanism, with random addition of substrates and products at the two sites. The formal mechanism of these enzymes is presented here as a mixture of rapid equilibrium random segments connected by a steady-state segment, i.e. a rapid-equilibrium random (two-site) hybrid ping-pong mechanism. Such a mechanism is likely to be operative in a variety of oxidative enzymes containing multiple redox-active prosthetic groups.

Duke Scholars

Published In

Eur J Biochem

DOI

ISSN

0014-2956

Publication Date

March 1980

Volume

105

Issue

1

Start / End Page

81 / 84

Location

England

Related Subject Headings

  • Xanthine Dehydrogenase
  • Liver
  • Kinetics
  • Ketone Oxidoreductases
  • Chickens
  • Biochemistry & Molecular Biology
  • Binding Sites
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3205 Medical biochemistry and metabolomics
 

Citation

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Coughlan, M. P., & Rajagopalan, K. V. (1980). The kinetic mechanism of xanthine dehydrogenase and related enzymes. Eur J Biochem, 105(1), 81–84. https://doi.org/10.1111/j.1432-1033.1980.tb04476.x
Coughlan, M. P., and K. V. Rajagopalan. “The kinetic mechanism of xanthine dehydrogenase and related enzymes.Eur J Biochem 105, no. 1 (March 1980): 81–84. https://doi.org/10.1111/j.1432-1033.1980.tb04476.x.
Coughlan MP, Rajagopalan KV. The kinetic mechanism of xanthine dehydrogenase and related enzymes. Eur J Biochem. 1980 Mar;105(1):81–4.
Coughlan, M. P., and K. V. Rajagopalan. “The kinetic mechanism of xanthine dehydrogenase and related enzymes.Eur J Biochem, vol. 105, no. 1, Mar. 1980, pp. 81–84. Pubmed, doi:10.1111/j.1432-1033.1980.tb04476.x.
Coughlan MP, Rajagopalan KV. The kinetic mechanism of xanthine dehydrogenase and related enzymes. Eur J Biochem. 1980 Mar;105(1):81–84.

Published In

Eur J Biochem

DOI

ISSN

0014-2956

Publication Date

March 1980

Volume

105

Issue

1

Start / End Page

81 / 84

Location

England

Related Subject Headings

  • Xanthine Dehydrogenase
  • Liver
  • Kinetics
  • Ketone Oxidoreductases
  • Chickens
  • Biochemistry & Molecular Biology
  • Binding Sites
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3205 Medical biochemistry and metabolomics