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Identification of a molybdopterin-containing molybdenum cofactor in xanthine dehydrogenase from Pseudomonas aeruginosa.

Publication ,  Journal Article
Johnson, JL; Chaudhury, M; Rajagopalan, KV
Published in: Biofactors
June 1991

Xanthine dehydrogenase has been purified from Pseudomonas aeruginosa cultured on a rich medium and induced with hypoxanthine. The enzyme was shown to contain FAD, iron sulfur centers and a molybdenum cofactor as prosthetic groups. Analysis of the molybdenum cofactor in this enzyme has revealed that the cofactor contains molybdopterin (MPT) rather than molybdopterin guanine dinucleotide or molybdopterin cytosine dinucleotide which have previously been identified in a number of molybdoenzymes of bacterial origin. The pterin cofactor in P.aeruginosa xanthine dehydrogenase was alkylated and the resulting product was identified as dicarboxamidomethyl molybdopterin. In addition, the pterin released from the enzyme by denaturation with guanidine-HCl was found to chromatograph on Sephadex G-15 with an apparent molecular weight of 350. These results document the first example of a bacterial enzyme with a molybdenum cofactor comprising molybdopterin and the metal only.

Duke Scholars

Published In

Biofactors

ISSN

0951-6433

Publication Date

June 1991

Volume

3

Issue

2

Start / End Page

103 / 107

Location

Netherlands

Related Subject Headings

  • Xanthine Dehydrogenase
  • Spectrum Analysis
  • Pteridines
  • Pseudomonas aeruginosa
  • Molybdenum Cofactors
  • Molybdenum
  • Molecular Structure
  • Metalloproteins
  • Flavin-Adenine Dinucleotide
  • Electron Spin Resonance Spectroscopy
 

Citation

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MLA
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Johnson, J. L., Chaudhury, M., & Rajagopalan, K. V. (1991). Identification of a molybdopterin-containing molybdenum cofactor in xanthine dehydrogenase from Pseudomonas aeruginosa. Biofactors, 3(2), 103–107.
Johnson, J. L., M. Chaudhury, and K. V. Rajagopalan. “Identification of a molybdopterin-containing molybdenum cofactor in xanthine dehydrogenase from Pseudomonas aeruginosa.Biofactors 3, no. 2 (June 1991): 103–7.
Johnson, J. L., et al. “Identification of a molybdopterin-containing molybdenum cofactor in xanthine dehydrogenase from Pseudomonas aeruginosa.Biofactors, vol. 3, no. 2, June 1991, pp. 103–07.
Journal cover image

Published In

Biofactors

ISSN

0951-6433

Publication Date

June 1991

Volume

3

Issue

2

Start / End Page

103 / 107

Location

Netherlands

Related Subject Headings

  • Xanthine Dehydrogenase
  • Spectrum Analysis
  • Pteridines
  • Pseudomonas aeruginosa
  • Molybdenum Cofactors
  • Molybdenum
  • Molecular Structure
  • Metalloproteins
  • Flavin-Adenine Dinucleotide
  • Electron Spin Resonance Spectroscopy