Skip to main content
Journal cover image

Molybdopterin guanine dinucleotide: a modified form of molybdopterin identified in the molybdenum cofactor of dimethyl sulfoxide reductase from Rhodobacter sphaeroides forma specialis denitrificans.

Publication ,  Journal Article
Johnson, JL; Bastian, NR; Rajagopalan, KV
Published in: Proc Natl Acad Sci U S A
April 1990

The nature of molybdenum cofactor in the bacterial enzyme dimethyl sulfoxide reductase has been investigated by application of alkylation conditions that convert the molybdenum cofactor in chicken liver sulfite oxidase and milk xanthine oxidase to the stable, well-characterized derivative [di(carboxamidomethyl)]molybdopterin. The alkylated pterin obtained from dimethyl sulfoxide reductase was shown to be a modified form of alkylated molybdopterin with increased absorption in the 250-nm region of the spectrum and altered chromatographic behavior. The complex alkylated pterin was resolved into two components by treatment with nucleotide pyrophosphatase. These were identified as di(carboxamidomethyl)molybdopterin and GMP by their absorption spectra, coelution with standard compounds, and by further degradation by alkaline phosphatase to dephospho [di(carboxamidomethyl)]molybdopterin and guanosine. The GMP moiety was sensitive to periodate, identifying it as the 5' isomer. Chemical analysis of the intact alkylated pterin showed the presence of two phosphate residues per pterin. These results established that the pterin isolated from dimethyl sulfoxide reductase contains the phosphoric anhydride of molybdopterin and 5'-GMP, which is designated molybdopterin guanine dinucleotide.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 1990

Volume

87

Issue

8

Start / End Page

3190 / 3194

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Rhodobacter sphaeroides
  • Pterins
  • Pteridines
  • Oxidoreductases
  • Molybdenum Cofactors
  • Molybdenum
  • Metalloproteins
  • Iron-Sulfur Proteins
  • Guanine Nucleotides
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Johnson, J. L., Bastian, N. R., & Rajagopalan, K. V. (1990). Molybdopterin guanine dinucleotide: a modified form of molybdopterin identified in the molybdenum cofactor of dimethyl sulfoxide reductase from Rhodobacter sphaeroides forma specialis denitrificans. Proc Natl Acad Sci U S A, 87(8), 3190–3194. https://doi.org/10.1073/pnas.87.8.3190
Johnson, J. L., N. R. Bastian, and K. V. Rajagopalan. “Molybdopterin guanine dinucleotide: a modified form of molybdopterin identified in the molybdenum cofactor of dimethyl sulfoxide reductase from Rhodobacter sphaeroides forma specialis denitrificans.Proc Natl Acad Sci U S A 87, no. 8 (April 1990): 3190–94. https://doi.org/10.1073/pnas.87.8.3190.
Johnson, J. L., et al. “Molybdopterin guanine dinucleotide: a modified form of molybdopterin identified in the molybdenum cofactor of dimethyl sulfoxide reductase from Rhodobacter sphaeroides forma specialis denitrificans.Proc Natl Acad Sci U S A, vol. 87, no. 8, Apr. 1990, pp. 3190–94. Pubmed, doi:10.1073/pnas.87.8.3190.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

April 1990

Volume

87

Issue

8

Start / End Page

3190 / 3194

Location

United States

Related Subject Headings

  • Spectrophotometry
  • Rhodobacter sphaeroides
  • Pterins
  • Pteridines
  • Oxidoreductases
  • Molybdenum Cofactors
  • Molybdenum
  • Metalloproteins
  • Iron-Sulfur Proteins
  • Guanine Nucleotides