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Intratumoral heterogeneity in primary breast carcinoma: study of concurrent parameters.

Publication ,  Journal Article
Dodd, LG; Kerns, BJ; Dodge, RK; Layfield, LJ
Published in: J Surg Oncol
April 1997

BACKGROUND AND OBJECTIVE: Intratumoral heterogeneity for prognostic factors (ploidy, proliferation, hormone receptor positivity) has been demonstrated in primary breast carcinoma by both flow cytometric and image analysis methods. Previously, heterogeneity in tumors had been demonstrated for only singular parameters. Our objective, using maps of tumors in which discrete regions can be analyzed simultaneously for DNA index (DI) and proliferative activity, was to demonstrate heterogeneity with respect to two parameters and to determine whether any interparametric relationships existed. METHODS: We analyzed 25 cases of archived, paraffin-embedded breast carcinoma (ductal) for Feulgen stain DNA analysis and MIB-1 immunohistochemistry using the CAS 200 Image Cytometer. For each tumor, four discrete regions were analyzed including tumor-host tissue interface sectors. RESULTS: Of 25 cases, 19 (76%) were homogeneously diploid or near-diploid aneuploid, and 6 (24%) were heterogeneous. Within the heterogeneous group, all cases had at least one diploid and one or more aneuploid populations from separate discrete regions. Five of six DI heterogeneous tumors displayed diploid values for the overall measurements of the respective tumors, based on analysis of 200 or more nuclei. Eight of 25 cases (32%) showed significant measurable variation for MIB-1 proliferative activity in various sectors of tumor. All the MIB-1 heterogeneous tumors, with one exception, were homogeneously diploid. CONCLUSIONS: These findings demonstrate that (1) heterogeneity is present with respect to DI and proliferative activity in breast carcinoma and is relatively common, (2) tumors homogeneous for one parameter may be heterogeneous for another, and (3) heterogeneity for proliferative activity is more common in homogeneously diploid tumors than in heterogeneous/aneuploid tumors.

Duke Scholars

Published In

J Surg Oncol

DOI

ISSN

0022-4790

Publication Date

April 1997

Volume

64

Issue

4

Start / End Page

280 / 287

Location

United States

Related Subject Headings

  • Prognosis
  • Ploidies
  • Oncology & Carcinogenesis
  • Immunohistochemistry
  • Humans
  • Flow Cytometry
  • Female
  • DNA, Neoplasm
  • Cell Division
  • Carcinoma, Ductal, Breast
 

Citation

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Dodd, L. G., Kerns, B. J., Dodge, R. K., & Layfield, L. J. (1997). Intratumoral heterogeneity in primary breast carcinoma: study of concurrent parameters. J Surg Oncol, 64(4), 280–287. https://doi.org/10.1002/(sici)1096-9098(199704)64:4<280::aid-jso6>3.0.co;2-5
Dodd, L. G., B. J. Kerns, R. K. Dodge, and L. J. Layfield. “Intratumoral heterogeneity in primary breast carcinoma: study of concurrent parameters.J Surg Oncol 64, no. 4 (April 1997): 280–87. https://doi.org/10.1002/(sici)1096-9098(199704)64:4<280::aid-jso6>3.0.co;2-5.
Dodd LG, Kerns BJ, Dodge RK, Layfield LJ. Intratumoral heterogeneity in primary breast carcinoma: study of concurrent parameters. J Surg Oncol. 1997 Apr;64(4):280–7.
Dodd, L. G., et al. “Intratumoral heterogeneity in primary breast carcinoma: study of concurrent parameters.J Surg Oncol, vol. 64, no. 4, Apr. 1997, pp. 280–87. Pubmed, doi:10.1002/(sici)1096-9098(199704)64:4<280::aid-jso6>3.0.co;2-5.
Dodd LG, Kerns BJ, Dodge RK, Layfield LJ. Intratumoral heterogeneity in primary breast carcinoma: study of concurrent parameters. J Surg Oncol. 1997 Apr;64(4):280–287.
Journal cover image

Published In

J Surg Oncol

DOI

ISSN

0022-4790

Publication Date

April 1997

Volume

64

Issue

4

Start / End Page

280 / 287

Location

United States

Related Subject Headings

  • Prognosis
  • Ploidies
  • Oncology & Carcinogenesis
  • Immunohistochemistry
  • Humans
  • Flow Cytometry
  • Female
  • DNA, Neoplasm
  • Cell Division
  • Carcinoma, Ductal, Breast