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Prevalence of non-A, non-B hepatitis/hepatitis C virus antibody in laboratory quality-assurance sera.

Publication ,  Journal Article
Dodd, LG; McBride, JH; Gitnick, GL; Howanitz, PJ; Rodgerson, DO
Published in: Clin Chem
June 1991

Quality-assurance sera (QAS) are prepared from pooled sera composed of thousands of individual donations. Previous studies documented that a substantial percentage of individual QAS test positive for viral disease markers, including antibodies to human immunodeficiency virus and to hepatitis B surface antigen. We tested 239 QAS from various proficiency programs and commercial sources to determine the prevalence of hepatitis C virus (HCV) antibody. We tested samples for anti-HCV by using an enzyme immunoassay (EIA; Abbott Labs.) and an enzyme-linked immunosorbent assay (ELISA; Ortho Diagnostics). We observed an overall positive rate of 49% by one or both assays in all categories of sera tested. In addition, we found a greater rate of positivity (58%) in proficiency program samples than in commercial samples (43%). We found discrepant results between the two assays for 15 of 239 samples (6%). In the discrepant samples, the EIA result was positive, whereas the ELISA result was negative. Anti-HCV positivity in QAS has important implications for laboratory personnel handling these samples.

Duke Scholars

Published In

Clin Chem

ISSN

0009-9147

Publication Date

June 1991

Volume

37

Issue

6

Start / End Page

797 / 803

Location

England

Related Subject Headings

  • Quality Assurance, Health Care
  • Laboratories
  • Immunoenzyme Techniques
  • Humans
  • Hepacivirus
  • General Clinical Medicine
  • Enzyme-Linked Immunosorbent Assay
  • Blood
  • Antibodies, Viral
  • 1103 Clinical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dodd, L. G., McBride, J. H., Gitnick, G. L., Howanitz, P. J., & Rodgerson, D. O. (1991). Prevalence of non-A, non-B hepatitis/hepatitis C virus antibody in laboratory quality-assurance sera. Clin Chem, 37(6), 797–803.
Dodd, L. G., J. H. McBride, G. L. Gitnick, P. J. Howanitz, and D. O. Rodgerson. “Prevalence of non-A, non-B hepatitis/hepatitis C virus antibody in laboratory quality-assurance sera.Clin Chem 37, no. 6 (June 1991): 797–803.
Dodd LG, McBride JH, Gitnick GL, Howanitz PJ, Rodgerson DO. Prevalence of non-A, non-B hepatitis/hepatitis C virus antibody in laboratory quality-assurance sera. Clin Chem. 1991 Jun;37(6):797–803.
Dodd, L. G., et al. “Prevalence of non-A, non-B hepatitis/hepatitis C virus antibody in laboratory quality-assurance sera.Clin Chem, vol. 37, no. 6, June 1991, pp. 797–803.
Dodd LG, McBride JH, Gitnick GL, Howanitz PJ, Rodgerson DO. Prevalence of non-A, non-B hepatitis/hepatitis C virus antibody in laboratory quality-assurance sera. Clin Chem. 1991 Jun;37(6):797–803.

Published In

Clin Chem

ISSN

0009-9147

Publication Date

June 1991

Volume

37

Issue

6

Start / End Page

797 / 803

Location

England

Related Subject Headings

  • Quality Assurance, Health Care
  • Laboratories
  • Immunoenzyme Techniques
  • Humans
  • Hepacivirus
  • General Clinical Medicine
  • Enzyme-Linked Immunosorbent Assay
  • Blood
  • Antibodies, Viral
  • 1103 Clinical Sciences