![Journal cover image](https://secure.syndetics.com/index.aspx?isbn=/lc.gif&issn=0950-382X&client=dukeuniv)
Tn5-mediated bleomycin resistance in Escherichia coli requires the expression of host genes.
Publication
, Journal Article
Blot, M; Heitman, J; Arber, W
Published in: Mol Microbiol
June 1993
The transposon Tn5 expresses a gene, ble, whose product increases the viability of Escherichia coli and also confers resistance to the DNA-cleaving antibiotic bleomycin and the DNA-alkylating agent ethylmethanesulphonate. We find that the Ble protein induces expression of an alkylation inducible gene, aidC, and that both the AidC gene product and DNA polymerase I are required for Ble to confer bleomycin resistance. These findings support models in which Ble enhances DNA repair and suggest that Tn5 confers a fitness advantage to the host bacterium by increasing the repair of spontaneous DNA lesions. Such co-operation between a transposon and its host suggests that Tn5 is a symbiotic rather than a selfish DNA element.
Duke Scholars
Published In
Mol Microbiol
DOI
ISSN
0950-382X
Publication Date
June 1993
Volume
8
Issue
6
Start / End Page
1017 / 1024
Location
England
Related Subject Headings
- Transcription Factors
- O(6)-Methylguanine-DNA Methyltransferase
- N-Glycosyl Hydrolases
- Microbiology
- Genes, Bacterial
- Gene Expression Regulation, Bacterial
- Ethyl Methanesulfonate
- Escherichia coli Proteins
- Escherichia coli
- Drug Resistance, Microbial
Citation
APA
Chicago
ICMJE
MLA
NLM
Blot, M., Heitman, J., & Arber, W. (1993). Tn5-mediated bleomycin resistance in Escherichia coli requires the expression of host genes. Mol Microbiol, 8(6), 1017–1024. https://doi.org/10.1111/j.1365-2958.1993.tb01646.x
Blot, M., J. Heitman, and W. Arber. “Tn5-mediated bleomycin resistance in Escherichia coli requires the expression of host genes.” Mol Microbiol 8, no. 6 (June 1993): 1017–24. https://doi.org/10.1111/j.1365-2958.1993.tb01646.x.
Blot M, Heitman J, Arber W. Tn5-mediated bleomycin resistance in Escherichia coli requires the expression of host genes. Mol Microbiol. 1993 Jun;8(6):1017–24.
Blot, M., et al. “Tn5-mediated bleomycin resistance in Escherichia coli requires the expression of host genes.” Mol Microbiol, vol. 8, no. 6, June 1993, pp. 1017–24. Pubmed, doi:10.1111/j.1365-2958.1993.tb01646.x.
Blot M, Heitman J, Arber W. Tn5-mediated bleomycin resistance in Escherichia coli requires the expression of host genes. Mol Microbiol. 1993 Jun;8(6):1017–1024.
![Journal cover image](https://secure.syndetics.com/index.aspx?isbn=/lc.gif&issn=0950-382X&client=dukeuniv)
Published In
Mol Microbiol
DOI
ISSN
0950-382X
Publication Date
June 1993
Volume
8
Issue
6
Start / End Page
1017 / 1024
Location
England
Related Subject Headings
- Transcription Factors
- O(6)-Methylguanine-DNA Methyltransferase
- N-Glycosyl Hydrolases
- Microbiology
- Genes, Bacterial
- Gene Expression Regulation, Bacterial
- Ethyl Methanesulfonate
- Escherichia coli Proteins
- Escherichia coli
- Drug Resistance, Microbial