SOS induction as an in vivo assay of enzyme-DNA interactions.
Publication
, Journal Article
Heitman, J; Model, P
Published in: Gene
July 15, 1991
We have constructed strains which are convenient and sensitive indicators of DNA damage and describe their use. These strains utilize an SOS::lac Z fusion constructed by Kenyon and Walker [Proc. Natl. Acad. Sci. USA 77 (1980) 2819-2823] and respond to DNA damage by producing beta-galactosidase. They can be used to characterize restriction systems and screen for restriction endonuclease mutants. Applications include the study of other enzymes involved in DNA metabolism, such as DNA methyltransferases, topoisomerases, recombinases, and DNA replication and repair enzymes.
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Published In
Gene
DOI
ISSN
0378-1119
Publication Date
July 15, 1991
Volume
103
Issue
1
Start / End Page
1 / 9
Location
Netherlands
Related Subject Headings
- beta-Galactosidase
- SOS Response, Genetics
- Recombinant Fusion Proteins
- Mutation
- Gene Expression Regulation, Bacterial
- Escherichia coli
- Developmental Biology
- Deoxyribonuclease EcoRI
- DNA-Binding Proteins
- DNA Modification Methylases
Citation
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ICMJE
MLA
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Heitman, J., & Model, P. (1991). SOS induction as an in vivo assay of enzyme-DNA interactions. Gene, 103(1), 1–9. https://doi.org/10.1016/0378-1119(91)90383-m
Heitman, J., and P. Model. “SOS induction as an in vivo assay of enzyme-DNA interactions.” Gene 103, no. 1 (July 15, 1991): 1–9. https://doi.org/10.1016/0378-1119(91)90383-m.
Heitman J, Model P. SOS induction as an in vivo assay of enzyme-DNA interactions. Gene. 1991 Jul 15;103(1):1–9.
Heitman, J., and P. Model. “SOS induction as an in vivo assay of enzyme-DNA interactions.” Gene, vol. 103, no. 1, July 1991, pp. 1–9. Pubmed, doi:10.1016/0378-1119(91)90383-m.
Heitman J, Model P. SOS induction as an in vivo assay of enzyme-DNA interactions. Gene. 1991 Jul 15;103(1):1–9.
Published In
Gene
DOI
ISSN
0378-1119
Publication Date
July 15, 1991
Volume
103
Issue
1
Start / End Page
1 / 9
Location
Netherlands
Related Subject Headings
- beta-Galactosidase
- SOS Response, Genetics
- Recombinant Fusion Proteins
- Mutation
- Gene Expression Regulation, Bacterial
- Escherichia coli
- Developmental Biology
- Deoxyribonuclease EcoRI
- DNA-Binding Proteins
- DNA Modification Methylases