Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae.

Publication ,  Journal Article
Lorenz, MC; Pan, X; Harashima, T; Cardenas, ME; Xue, Y; Hirsch, JP; Heitman, J
Published in: Genetics
February 2000

Pseudohyphal differentiation in the budding yeast Saccharomyces cerevisiae is induced in diploid cells in response to nitrogen starvation and abundant fermentable carbon source. Filamentous growth requires at least two signaling pathways: the pheromone responsive MAP kinase cascade and the Gpa2p-cAMP-PKA signaling pathway. Recent studies have established a physical and functional link between the Galpha protein Gpa2 and the G protein-coupled receptor homolog Gpr1. We report here that the Gpr1 receptor is required for filamentous and haploid invasive growth and regulates expression of the cell surface flocculin Flo11. Epistasis analysis supports a model in which the Gpr1 receptor regulates pseudohyphal growth via the Gpa2p-cAMP-PKA pathway and independently of both the MAP kinase cascade and the PKA related kinase Sch9. Genetic and physiological studies indicate that the Gpr1 receptor is activated by glucose and other structurally related sugars. Because expression of the GPR1 gene is known to be induced by nitrogen starvation, the Gpr1 receptor may serve as a dual sensor of abundant carbon source (sugar ligand) and nitrogen starvation. In summary, our studies reveal a novel G protein-coupled receptor senses nutrients and regulates the dimorphic transition to filamentous growth via a Galpha protein-cAMP-PKA signal transduction cascade.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

February 2000

Volume

154

Issue

2

Start / End Page

609 / 622

Location

United States

Related Subject Headings

  • Signal Transduction
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Receptors, G-Protein-Coupled
  • Receptors, Cell Surface
  • Membrane Proteins
  • Membrane Glycoproteins
  • Haploidy
  • Gene Expression Regulation, Fungal
  • Fungal Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lorenz, M. C., Pan, X., Harashima, T., Cardenas, M. E., Xue, Y., Hirsch, J. P., & Heitman, J. (2000). The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Genetics, 154(2), 609–622. https://doi.org/10.1093/genetics/154.2.609
Lorenz, M. C., X. Pan, T. Harashima, M. E. Cardenas, Y. Xue, J. P. Hirsch, and J. Heitman. “The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae.Genetics 154, no. 2 (February 2000): 609–22. https://doi.org/10.1093/genetics/154.2.609.
Lorenz MC, Pan X, Harashima T, Cardenas ME, Xue Y, Hirsch JP, et al. The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Genetics. 2000 Feb;154(2):609–22.
Lorenz, M. C., et al. “The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae.Genetics, vol. 154, no. 2, Feb. 2000, pp. 609–22. Pubmed, doi:10.1093/genetics/154.2.609.
Lorenz MC, Pan X, Harashima T, Cardenas ME, Xue Y, Hirsch JP, Heitman J. The G protein-coupled receptor gpr1 is a nutrient sensor that regulates pseudohyphal differentiation in Saccharomyces cerevisiae. Genetics. 2000 Feb;154(2):609–622.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

February 2000

Volume

154

Issue

2

Start / End Page

609 / 622

Location

United States

Related Subject Headings

  • Signal Transduction
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Receptors, G-Protein-Coupled
  • Receptors, Cell Surface
  • Membrane Proteins
  • Membrane Glycoproteins
  • Haploidy
  • Gene Expression Regulation, Fungal
  • Fungal Proteins