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Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis.

Publication ,  Journal Article
Paisley, JW; Mirrett, S; Lauer, BA; Roe, M; Reller, LB
Published in: J Clin Microbiol
January 1982

To determine the value of direct dark-field microscopy for diagnosing enteritis due to Campylobacter fetus subsp. jejuni, we examined 1,377 human fecal specimens for bacteria with typical Campylobacter darting motility, leukocytes, and erythrocytes. Eighty-four specimens (6.1%) grew C.fetus subsp. jejuni. Of the 48 specimens showing Campylobacter motility, 30 (62%) grew C. fetus subsp. jejuni. The sensitivity, specificity, and predictive value of observing Campylobacter motility were 36%, 99%, and 62%, respectively. The predictive value of detecting Campylobacter motility was improved if the specimens were diarrheal (23 of 31, 74%), leukocytes were present (25 of 33, 76%), erythrocytes were present (22 of 27, 81%), or if all of the above findings were present (18 of 20, 90%). The sensitivity of detecting Campylobacter darting motility was highest if specimens were examined within 2 h of arrival in the laboratory (15 of 30, 50%) as opposed to after 2 h (15 of 53, 28%; P less than 0.01). Prompt dark-field microscopic examination of diarrheal stool specimens is valuable for the presumptive diagnosis of Campylobacter enteritis.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

January 1982

Volume

15

Issue

1

Start / End Page

61 / 63

Location

United States

Related Subject Headings

  • Microscopy
  • Microbiology
  • Humans
  • Feces
  • Enteritis
  • Campylobacter fetus
  • Campylobacter Infections
  • Bacteriological Techniques
  • 3207 Medical microbiology
  • 3202 Clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Paisley, J. W., Mirrett, S., Lauer, B. A., Roe, M., & Reller, L. B. (1982). Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis. J Clin Microbiol, 15(1), 61–63. https://doi.org/10.1128/jcm.15.1.61-63.1982
Paisley, J. W., S. Mirrett, B. A. Lauer, M. Roe, and L. B. Reller. “Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis.J Clin Microbiol 15, no. 1 (January 1982): 61–63. https://doi.org/10.1128/jcm.15.1.61-63.1982.
Paisley JW, Mirrett S, Lauer BA, Roe M, Reller LB. Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis. J Clin Microbiol. 1982 Jan;15(1):61–3.
Paisley, J. W., et al. “Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis.J Clin Microbiol, vol. 15, no. 1, Jan. 1982, pp. 61–63. Pubmed, doi:10.1128/jcm.15.1.61-63.1982.
Paisley JW, Mirrett S, Lauer BA, Roe M, Reller LB. Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis. J Clin Microbiol. 1982 Jan;15(1):61–63.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

January 1982

Volume

15

Issue

1

Start / End Page

61 / 63

Location

United States

Related Subject Headings

  • Microscopy
  • Microbiology
  • Humans
  • Feces
  • Enteritis
  • Campylobacter fetus
  • Campylobacter Infections
  • Bacteriological Techniques
  • 3207 Medical microbiology
  • 3202 Clinical sciences