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Development of a standardized screening method for detection of vancomycin-resistant enterococci.

Publication ,  Journal Article
Swenson, JM; Clark, NC; Ferraro, MJ; Sahm, DF; Doern, G; Pfaller, MA; Reller, LB; Weinstein, MP; Zabransky, RJ; Tenover, FC
Published in: J Clin Microbiol
July 1994

The incidence of vancomycin resistance among enterococci is increasing in the United States and elsewhere in the world, but automated susceptibility testing methods have difficulty detecting resistance expressed by certain strains. The agar screening method described by Willey et al. (B. M. Willey, B. N. Kreiswirth, A. E. Simor, G. Williams, S. R. Scriver, A. Phillips, and D. E. Low, J. Clin. Microbiol. 30:1621-1624, 1992) has been proposed as a reliable method for confirming vancomycin resistance. In this study, we investigated various parameters associated with the agar screening method and, on the basis of the findings, established optimum testing conditions for the method. First, to evaluate media and vancomycin concentrations, one laboratory used Mueller-Hinton and brain heart infusion agars supplemented with 4, 6, and 8 micrograms of vancomycin per ml to test 100 genetically characterized enterococcal strains. On the basis of the results obtained, brain heart infusion agar supplemented with 6 micrograms of vancomycin per ml was selected for further study. Subsequently, eight laboratories used the medium to test both reference and clinical isolates. There was very good performance with the reference strains and, among 158 clinical isolates tested, the method demonstrated sensitivity and specificity of 100% and from 96 to 99%, respectively.

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Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

July 1994

Volume

32

Issue

7

Start / End Page

1700 / 1704

Location

United States

Related Subject Headings

  • Vancomycin
  • Species Specificity
  • Sensitivity and Specificity
  • Microbiology
  • Microbial Sensitivity Tests
  • Enterococcus
  • Drug Resistance, Microbial
  • Culture Media
  • 3207 Medical microbiology
  • 3202 Clinical sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Swenson, J. M., Clark, N. C., Ferraro, M. J., Sahm, D. F., Doern, G., Pfaller, M. A., … Tenover, F. C. (1994). Development of a standardized screening method for detection of vancomycin-resistant enterococci. J Clin Microbiol, 32(7), 1700–1704. https://doi.org/10.1128/jcm.32.7.1700-1704.1994
Swenson, J. M., N. C. Clark, M. J. Ferraro, D. F. Sahm, G. Doern, M. A. Pfaller, L. B. Reller, M. P. Weinstein, R. J. Zabransky, and F. C. Tenover. “Development of a standardized screening method for detection of vancomycin-resistant enterococci.J Clin Microbiol 32, no. 7 (July 1994): 1700–1704. https://doi.org/10.1128/jcm.32.7.1700-1704.1994.
Swenson JM, Clark NC, Ferraro MJ, Sahm DF, Doern G, Pfaller MA, et al. Development of a standardized screening method for detection of vancomycin-resistant enterococci. J Clin Microbiol. 1994 Jul;32(7):1700–4.
Swenson, J. M., et al. “Development of a standardized screening method for detection of vancomycin-resistant enterococci.J Clin Microbiol, vol. 32, no. 7, July 1994, pp. 1700–04. Pubmed, doi:10.1128/jcm.32.7.1700-1704.1994.
Swenson JM, Clark NC, Ferraro MJ, Sahm DF, Doern G, Pfaller MA, Reller LB, Weinstein MP, Zabransky RJ, Tenover FC. Development of a standardized screening method for detection of vancomycin-resistant enterococci. J Clin Microbiol. 1994 Jul;32(7):1700–1704.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

July 1994

Volume

32

Issue

7

Start / End Page

1700 / 1704

Location

United States

Related Subject Headings

  • Vancomycin
  • Species Specificity
  • Sensitivity and Specificity
  • Microbiology
  • Microbial Sensitivity Tests
  • Enterococcus
  • Drug Resistance, Microbial
  • Culture Media
  • 3207 Medical microbiology
  • 3202 Clinical sciences