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Development of resistance in Pseudomonas aeruginosa to imipenem, norfloxacin, and ciprofloxacin during therapy: proof provided by typing with a DNA probe.

Publication ,  Journal Article
Ogle, JW; Reller, LB; Vasil, ML
Published in: J Infect Dis
April 1988

Differentiating reinfection from the acquisition of resistance in strains of Pseudomonas aeruginosa after antimicrobial therapy is difficult because currently used epidemiological markers are not stable genetic markers. We previously established that a 741-base pair PstI-NruI restriction fragment upstream from the Exotoxin A structural gene is a sensitive, specific, and stable epidemiological marker for P. aeruginosa. Therefore, we used this fragment as a probe in Southern hybridization to compare pre- and post-therapy isolates of P. aeruginosa. The susceptible and resistant pairs were recovered from multiple sources (including sputum, blood, and urine) from patients treated with various doses of imipenem (n = 15), norfloxacin (n = 6), and ciprofloxacin (n = 4). Southern blot analysis showed identity between the pre- and post-therapy isolates in 23 of the 25 pairs. In the majority of pairs studied, failure to eradicate P. aeruginosa after therapy with imipenem, norfloxacin, and ciprofloxacin was due to the development of resistance rather than to reinfection.

Duke Scholars

Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

April 1988

Volume

157

Issue

4

Start / End Page

743 / 748

Location

United States

Related Subject Headings

  • Thienamycins
  • Pseudomonas aeruginosa
  • Plasmids
  • Norfloxacin
  • Microbiology
  • Imipenem
  • Drug Resistance, Microbial
  • DNA, Bacterial
  • Ciprofloxacin
  • 42 Health sciences
 

Citation

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MLA
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Ogle, J. W., Reller, L. B., & Vasil, M. L. (1988). Development of resistance in Pseudomonas aeruginosa to imipenem, norfloxacin, and ciprofloxacin during therapy: proof provided by typing with a DNA probe. J Infect Dis, 157(4), 743–748. https://doi.org/10.1093/infdis/157.4.743
Ogle, J. W., L. B. Reller, and M. L. Vasil. “Development of resistance in Pseudomonas aeruginosa to imipenem, norfloxacin, and ciprofloxacin during therapy: proof provided by typing with a DNA probe.J Infect Dis 157, no. 4 (April 1988): 743–48. https://doi.org/10.1093/infdis/157.4.743.
Ogle, J. W., et al. “Development of resistance in Pseudomonas aeruginosa to imipenem, norfloxacin, and ciprofloxacin during therapy: proof provided by typing with a DNA probe.J Infect Dis, vol. 157, no. 4, Apr. 1988, pp. 743–48. Pubmed, doi:10.1093/infdis/157.4.743.
Journal cover image

Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

April 1988

Volume

157

Issue

4

Start / End Page

743 / 748

Location

United States

Related Subject Headings

  • Thienamycins
  • Pseudomonas aeruginosa
  • Plasmids
  • Norfloxacin
  • Microbiology
  • Imipenem
  • Drug Resistance, Microbial
  • DNA, Bacterial
  • Ciprofloxacin
  • 42 Health sciences