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Identification of Histoplasma capsulatum from culture extracts by real-time PCR.

Publication ,  Journal Article
Martagon-Villamil, J; Shrestha, N; Sholtis, M; Isada, CM; Hall, GS; Bryne, T; Lodge, BA; Reller, LB; Procop, GW
Published in: J Clin Microbiol
March 2003

We designed and tested a real-time LightCycler PCR assay for Histoplasma capsulatum that correctly identified the 34 H. capsulatum isolates in a battery of 107 fungal isolates tested and also detected H. capsulatum in clinical specimens from three patients that were culture positive for this organism.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

March 2003

Volume

41

Issue

3

Start / End Page

1295 / 1298

Location

United States

Related Subject Headings

  • Polymerase Chain Reaction
  • Microbiology
  • Microbiological Techniques
  • Humans
  • Histoplasma
  • DNA, Fungal
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
 

Citation

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ICMJE
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Martagon-Villamil, J., Shrestha, N., Sholtis, M., Isada, C. M., Hall, G. S., Bryne, T., … Procop, G. W. (2003). Identification of Histoplasma capsulatum from culture extracts by real-time PCR. J Clin Microbiol, 41(3), 1295–1298. https://doi.org/10.1128/JCM.41.3.1295-1298.2003
Martagon-Villamil, Jose, Nabin Shrestha, Mary Sholtis, Carlos M. Isada, Gerri S. Hall, Terry Bryne, Barbara A. Lodge, L Barth Reller, and Gary W. Procop. “Identification of Histoplasma capsulatum from culture extracts by real-time PCR.J Clin Microbiol 41, no. 3 (March 2003): 1295–98. https://doi.org/10.1128/JCM.41.3.1295-1298.2003.
Martagon-Villamil J, Shrestha N, Sholtis M, Isada CM, Hall GS, Bryne T, et al. Identification of Histoplasma capsulatum from culture extracts by real-time PCR. J Clin Microbiol. 2003 Mar;41(3):1295–8.
Martagon-Villamil, Jose, et al. “Identification of Histoplasma capsulatum from culture extracts by real-time PCR.J Clin Microbiol, vol. 41, no. 3, Mar. 2003, pp. 1295–98. Pubmed, doi:10.1128/JCM.41.3.1295-1298.2003.
Martagon-Villamil J, Shrestha N, Sholtis M, Isada CM, Hall GS, Bryne T, Lodge BA, Reller LB, Procop GW. Identification of Histoplasma capsulatum from culture extracts by real-time PCR. J Clin Microbiol. 2003 Mar;41(3):1295–1298.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

March 2003

Volume

41

Issue

3

Start / End Page

1295 / 1298

Location

United States

Related Subject Headings

  • Polymerase Chain Reaction
  • Microbiology
  • Microbiological Techniques
  • Humans
  • Histoplasma
  • DNA, Fungal
  • 3207 Medical microbiology
  • 3202 Clinical sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences