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Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled.

Publication ,  Journal Article
Sussman, DJ; Klingensmith, J; Salinas, P; Adams, PS; Nusse, R; Perrimon, N
Published in: Dev Biol
November 1994

In the Drosophila embryo dishevelled (dsh) function is required by target cells in order to respond to wingless (wg, the homolog of Wnt-1), demonstrating a role for dsh in Wnt signal transduction. We have isolated a mouse homolog of the Drosophila dsh segment polarity gene. The 695-amino-acid protein encoded by the mouse dishevelled gene (Dvl-1) shares 50% identity (65% similarity) with dsh. Similarity searches of protein and DNA data bases revealed that Dvl-1 encodes an otherwise novel polypeptide. While no functional motifs were identified, one region of Dvl-1 was found to be similar to a domain of discs large-1 (dlg), a Drosophila tumor suppressor gene. In the embryo, Dvl-1 is expressed in most tissues, with uniformly high levels in the central nervous system. From 7.5 days postcoitum Dvl-1 is expressed throughout the developing brain and spinal cord, including those regions expressing Wnt-1 and En. Expression of Dvl-1 in adult mice was found to be widespread, with brain and testis exhibiting the highest levels. The majority of Dvl-1 expression in the adult cerebellum is in the granular cell layer, similar to the pattern seen for engrailed-2 (En-2). Throughout postnatal development of the brain Dvl-1 is highly expressed in areas of high neuronal cell density.

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Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

November 1994

Volume

166

Issue

1

Start / End Page

73 / 86

Location

United States

Related Subject Headings

  • Testis
  • Spinal Cord
  • Species Specificity
  • Sequence Homology, Amino Acid
  • Restriction Mapping
  • RNA, Messenger
  • Proteins
  • Protein Biosynthesis
  • Polymerase Chain Reaction
  • Phosphoproteins
 

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Sussman, D. J., Klingensmith, J., Salinas, P., Adams, P. S., Nusse, R., & Perrimon, N. (1994). Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled. Dev Biol, 166(1), 73–86. https://doi.org/10.1006/dbio.1994.1297
Sussman, D. J., J. Klingensmith, P. Salinas, P. S. Adams, R. Nusse, and N. Perrimon. “Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled.Dev Biol 166, no. 1 (November 1994): 73–86. https://doi.org/10.1006/dbio.1994.1297.
Sussman DJ, Klingensmith J, Salinas P, Adams PS, Nusse R, Perrimon N. Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled. Dev Biol. 1994 Nov;166(1):73–86.
Sussman, D. J., et al. “Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled.Dev Biol, vol. 166, no. 1, Nov. 1994, pp. 73–86. Pubmed, doi:10.1006/dbio.1994.1297.
Sussman DJ, Klingensmith J, Salinas P, Adams PS, Nusse R, Perrimon N. Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled. Dev Biol. 1994 Nov;166(1):73–86.
Journal cover image

Published In

Dev Biol

DOI

ISSN

0012-1606

Publication Date

November 1994

Volume

166

Issue

1

Start / End Page

73 / 86

Location

United States

Related Subject Headings

  • Testis
  • Spinal Cord
  • Species Specificity
  • Sequence Homology, Amino Acid
  • Restriction Mapping
  • RNA, Messenger
  • Proteins
  • Protein Biosynthesis
  • Polymerase Chain Reaction
  • Phosphoproteins