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Divergent effects of forskolin on 3',5' cyclic adenosine monophosphate production and parathyroid hormone secretion.

Publication ,  Journal Article
Cantley, LK; Scott, DL; Cooper, CW; Mahaffee, DD; Leight, GS; Thomas, CG; Ontjes, DA
Published in: Calcif Tissue Int
January 1984

Forskolin, a diterpene which directly stimulates adenylate cyclase, markedly stimulated cAMP production in intact rat parathyroid glands and dispersed cells from hyperplastic and adenomatous human parathyroid tissues. Stimulation of cAMP production in human parathyroid adenomas occurred as early as 2 min and continued for at least 2 h; furthermore, a dose-response relationship was observed, with a maximal 80-fold cAMP response occurring at 100 microM forskolin. When PTH secretion by rat or human parathyroid tissues was studied at low (0.5 mM) and high (2.5 mM) extracellular Ca2+ in either the presence or absence of forskolin, no significant stimulation by forskolin was observed at 15 min, 1 h, and 2 h. When 10 human parathyroid specimens were studied with varying concentrations of forskolin at 1 mM Ca2+, 6 failed to show stimulation of PTH secretion and 4 showed modest but detectable increases in PTH that did not appear dose-related. We conclude that (1) at low and high Ca2+ levels, marked stimulation of cAMP production by forskolin can occur without a corresponding increase in PTH secretion; (2) inhibition of PTH secretion by high extracellular Ca2+ levels continues unchanged despite stimulation of cAMP production by forskolin; and (3) at intermediate Ca2+ levels (1.0 mM), PTH secretion is affected either minimally or not at all by forskolin in human hyperparathyroid tissue preparations. The marked stimulation of parathyroid adenylate cyclase by forskolin without concomitant increases in PTH secretion in the majority of tissues suggests that the level of cAMP production is not a primary or sufficient determinant of hormone secretion.

Duke Scholars

Published In

Calcif Tissue Int

DOI

ISSN

0171-967X

Publication Date

January 1984

Volume

36

Issue

1

Start / End Page

87 / 94

Location

United States

Related Subject Headings

  • Time Factors
  • Rats
  • Parathyroid Hormone
  • Parathyroid Glands
  • Humans
  • Endocrinology & Metabolism
  • Dose-Response Relationship, Drug
  • Diterpenes
  • Cyclic AMP
  • Colforsin
 

Citation

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ICMJE
MLA
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Cantley, L. K., Scott, D. L., Cooper, C. W., Mahaffee, D. D., Leight, G. S., Thomas, C. G., & Ontjes, D. A. (1984). Divergent effects of forskolin on 3',5' cyclic adenosine monophosphate production and parathyroid hormone secretion. Calcif Tissue Int, 36(1), 87–94. https://doi.org/10.1007/BF02405299
Cantley, L. K., D. L. Scott, C. W. Cooper, D. D. Mahaffee, G. S. Leight, C. G. Thomas, and D. A. Ontjes. “Divergent effects of forskolin on 3',5' cyclic adenosine monophosphate production and parathyroid hormone secretion.Calcif Tissue Int 36, no. 1 (January 1984): 87–94. https://doi.org/10.1007/BF02405299.
Cantley LK, Scott DL, Cooper CW, Mahaffee DD, Leight GS, Thomas CG, et al. Divergent effects of forskolin on 3',5' cyclic adenosine monophosphate production and parathyroid hormone secretion. Calcif Tissue Int. 1984 Jan;36(1):87–94.
Cantley, L. K., et al. “Divergent effects of forskolin on 3',5' cyclic adenosine monophosphate production and parathyroid hormone secretion.Calcif Tissue Int, vol. 36, no. 1, Jan. 1984, pp. 87–94. Pubmed, doi:10.1007/BF02405299.
Cantley LK, Scott DL, Cooper CW, Mahaffee DD, Leight GS, Thomas CG, Ontjes DA. Divergent effects of forskolin on 3',5' cyclic adenosine monophosphate production and parathyroid hormone secretion. Calcif Tissue Int. 1984 Jan;36(1):87–94.
Journal cover image

Published In

Calcif Tissue Int

DOI

ISSN

0171-967X

Publication Date

January 1984

Volume

36

Issue

1

Start / End Page

87 / 94

Location

United States

Related Subject Headings

  • Time Factors
  • Rats
  • Parathyroid Hormone
  • Parathyroid Glands
  • Humans
  • Endocrinology & Metabolism
  • Dose-Response Relationship, Drug
  • Diterpenes
  • Cyclic AMP
  • Colforsin