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Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase.

Publication ,  Journal Article
Kelly, JL; Greenleaf, AL; Lehman, IR
Published in: J Biol Chem
August 5, 1986

Antisera directed against the purified 145,000-dalton subunit of the Saccharomyces cerevisiae mitochondrial RNA polymerase have been used to immuno-screen a library of yeast genomic inserts constructed in the fusion protein expression vector, lambda gt11. A 4-kilobase pair yeast DNA fragment inserted into one of the recombinant bacteriophages appears to contain most or all of the gene that encodes the 145,000-dalton subunit.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

August 5, 1986

Volume

261

Issue

22

Start / End Page

10348 / 10351

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Nucleic Acid Hybridization
  • Mitochondria
  • Immunologic Techniques
  • Immune Sera
  • Epitopes
  • DNA-Directed RNA Polymerases
  • DNA, Recombinant
  • DNA, Fungal
  • Collodion
 

Citation

APA
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ICMJE
MLA
NLM
Kelly, J. L., Greenleaf, A. L., & Lehman, I. R. (1986). Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase. J Biol Chem, 261(22), 10348–10351.
Kelly, J. L., A. L. Greenleaf, and I. R. Lehman. “Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase.J Biol Chem 261, no. 22 (August 5, 1986): 10348–51.
Kelly JL, Greenleaf AL, Lehman IR. Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase. J Biol Chem. 1986 Aug 5;261(22):10348–51.
Kelly, J. L., et al. “Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase.J Biol Chem, vol. 261, no. 22, Aug. 1986, pp. 10348–51.
Kelly JL, Greenleaf AL, Lehman IR. Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase. J Biol Chem. 1986 Aug 5;261(22):10348–10351.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

August 5, 1986

Volume

261

Issue

22

Start / End Page

10348 / 10351

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Nucleic Acid Hybridization
  • Mitochondria
  • Immunologic Techniques
  • Immune Sera
  • Epitopes
  • DNA-Directed RNA Polymerases
  • DNA, Recombinant
  • DNA, Fungal
  • Collodion