Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Spontaneous delta- to beta-globin switching in K562 human leukemia cells.

Publication ,  Journal Article
Mookerjee, B; Arcasoy, MO; Atweh, GF
Published in: Blood
February 1, 1992

Previous analysis of the hemoglobin phenotype of the K562 human erythroleukemia cell line showed regulated expression of the epsilon-, zeta-, gamma-, alpha-, and delta-globin genes. Expression of the beta-globin genes has not been previously detected in this cell line. In this report, we describe the isolation of a variant of the K562 cell line that actively expresses beta-globin messenger RNA (mRNA) and polypeptide and shows greatly reduced expression of the delta-globin genes. This phenotype developed spontaneously in culture while two other K562 isolates grown under the same culture conditions have not undergone the same delta- to beta-globin switch. Analysis of this unique K562 variant shows that a construct containing a beta-globin promoter is quite active upon transient transfection into these cells. This finding suggests that the activation of the endogenous beta-globin genes results from changes in the trans-acting environment of these cells. The regulation of the beta-globin genes in this variant is characterized by a paradoxical decrease in the level of beta-globin mRNA after exposure to hemin. Other globin genes of this variant are appropriately regulated and show increased expression after hemin induction. Further study of this variant may shed light on mechanisms of gene regulation that are involved in hemoglobin switching.

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

February 1, 1992

Volume

79

Issue

3

Start / End Page

820 / 825

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Leukemia, Erythroblastic, Acute
  • In Vitro Techniques
  • Immunology
  • Humans
  • Hemin
  • Globins
  • Gene Expression Regulation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mookerjee, B., Arcasoy, M. O., & Atweh, G. F. (1992). Spontaneous delta- to beta-globin switching in K562 human leukemia cells. Blood, 79(3), 820–825.
Mookerjee, B., M. O. Arcasoy, and G. F. Atweh. “Spontaneous delta- to beta-globin switching in K562 human leukemia cells.Blood 79, no. 3 (February 1, 1992): 820–25.
Mookerjee B, Arcasoy MO, Atweh GF. Spontaneous delta- to beta-globin switching in K562 human leukemia cells. Blood. 1992 Feb 1;79(3):820–5.
Mookerjee, B., et al. “Spontaneous delta- to beta-globin switching in K562 human leukemia cells.Blood, vol. 79, no. 3, Feb. 1992, pp. 820–25.
Mookerjee B, Arcasoy MO, Atweh GF. Spontaneous delta- to beta-globin switching in K562 human leukemia cells. Blood. 1992 Feb 1;79(3):820–825.

Published In

Blood

ISSN

0006-4971

Publication Date

February 1, 1992

Volume

79

Issue

3

Start / End Page

820 / 825

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Leukemia, Erythroblastic, Acute
  • In Vitro Techniques
  • Immunology
  • Humans
  • Hemin
  • Globins
  • Gene Expression Regulation