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A novel role for erythropoietin during fibrin-induced wound-healing response.

Publication ,  Journal Article
Haroon, ZA; Amin, K; Jiang, X; Arcasoy, MO
Published in: Am J Pathol
September 2003

In this study, we investigated the role of the hematopoietic cytokine erythropoietin (EPO) during wound healing, the physiological response to tissue injury. We used an in vivo wound-healing assay (fibrin Z-chambers) consisting of fibrin-filled chambers implanted subcutaneously in rats. The fibrin inside the chambers is replaced by granulation tissue consisting of new blood vessels, macrophages and fibroblasts as part of the wound-healing response. Local, exogenous recombinant EPO administration into the fibrin matrix significantly increased granulation tissue formation in a dose-dependent manner. To investigate the physiological role of endogenous EPO during wound healing, we used soluble EPO receptor or anti-EPO monoclonal antibodies to neutralize EPO and observed dose-dependent inhibition of granulation tissue formation, consistent with an important role for EPO in the wound-healing cascade. The ability of recombinant EPO to promote wound healing was associated with a proangiogenic effect during granulation tissue formation. We also found abundant expression of EPO receptor protein in macrophages, cells that play a pivotal role during wound healing. Modulation of wound healing because of administration of recombinant EPO or inhibition of endogenous EPO-EPO receptor correlated with changes in levels of inducible nitric oxide synthase protein in granulation tissue. These data demonstrate a novel function for EPO by providing in vivo evidence for a physiological role during fibrin-induced wound healing.

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Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

September 2003

Volume

163

Issue

3

Start / End Page

993 / 1000

Location

United States

Related Subject Headings

  • Wounds and Injuries
  • Wound Healing
  • Solubility
  • Skin
  • Recombinant Proteins
  • Receptors, Erythropoietin
  • Rats, Inbred F344
  • Rats
  • Pathology
  • Nitric Oxide Synthase Type II
 

Citation

APA
Chicago
ICMJE
MLA
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Haroon, Z. A., Amin, K., Jiang, X., & Arcasoy, M. O. (2003). A novel role for erythropoietin during fibrin-induced wound-healing response. Am J Pathol, 163(3), 993–1000. https://doi.org/10.1016/S0002-9440(10)63459-1
Haroon, Zishan A., Khalid Amin, Xiaohong Jiang, and Murat O. Arcasoy. “A novel role for erythropoietin during fibrin-induced wound-healing response.Am J Pathol 163, no. 3 (September 2003): 993–1000. https://doi.org/10.1016/S0002-9440(10)63459-1.
Haroon ZA, Amin K, Jiang X, Arcasoy MO. A novel role for erythropoietin during fibrin-induced wound-healing response. Am J Pathol. 2003 Sep;163(3):993–1000.
Haroon, Zishan A., et al. “A novel role for erythropoietin during fibrin-induced wound-healing response.Am J Pathol, vol. 163, no. 3, Sept. 2003, pp. 993–1000. Pubmed, doi:10.1016/S0002-9440(10)63459-1.
Haroon ZA, Amin K, Jiang X, Arcasoy MO. A novel role for erythropoietin during fibrin-induced wound-healing response. Am J Pathol. 2003 Sep;163(3):993–1000.
Journal cover image

Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

September 2003

Volume

163

Issue

3

Start / End Page

993 / 1000

Location

United States

Related Subject Headings

  • Wounds and Injuries
  • Wound Healing
  • Solubility
  • Skin
  • Recombinant Proteins
  • Receptors, Erythropoietin
  • Rats, Inbred F344
  • Rats
  • Pathology
  • Nitric Oxide Synthase Type II