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Erythropoietin and erythropoietin receptor expression in head and neck cancer: relationship to tumor hypoxia.

Publication ,  Journal Article
Arcasoy, MO; Amin, K; Chou, S-C; Haroon, ZA; Varia, M; Raleigh, JA
Published in: Clin Cancer Res
January 1, 2005

PURPOSE: Erythropoietin, an oxygen-regulated glycoprotein hormone, is a hematopoietic cytokine that stimulates erythropoiesis by binding to its cellular receptor [erythropoietin receptor (EPOR)]. The recombinant form of human erythropoietin is used to prevent or treat anemia in cancer patients. However, in a recent randomized, placebo-controlled trial involving patients receiving curative radiotherapy for squamous cell carcinoma of the head and neck, erythropoietin treatment was associated with poorer locoregional progression-free survival. The purpose of our study was to determine whether EPOR and its ligand erythropoietin are expressed in primary head and neck cancer. We also investigated the hypothesis that erythropoietin expression in malignant cells may be associated with the presence of tumor hypoxia, an important factor involved in resistance to radiation treatment, tumor aggressiveness, and poor prognosis. EXPERIMENTAL DESIGN: Twenty-one patients received an i.v. infusion of the hypoxia marker pimonidazole hydrochloride before multiple tumor biopsies. Contiguous sections from 74 biopsies were analyzed by immunohistochemistry for EPOR and erythropoietin expression and pimonidazole binding. RESULTS: EPOR expression was present in tumor cells in 97% of the biopsies. Coexpression of erythropoietin was observed in 90% of biopsies. Erythropoietin and pimonidazole adduct staining did not always colocalize within tumors, but there was a significant positive correlation between levels of microregional erythropoietin expression and pimonidazole binding. CONCLUSIONS: The coexpression of erythropoietin and EPOR in tumor cells suggests that erythropoietin may potentially function as an autocrine or paracrine factor in head and neck cancer. The expression of the hypoxia-inducible protein erythropoietin in tumor cells correlates with levels of tumor hypoxia.

Duke Scholars

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

January 1, 2005

Volume

11

Issue

1

Start / End Page

20 / 27

Location

United States

Related Subject Headings

  • Time Factors
  • Recombinant Proteins
  • Receptors, Erythropoietin
  • Radiation-Sensitizing Agents
  • Prognosis
  • Oncology & Carcinogenesis
  • Nitroimidazoles
  • Ligands
  • Immunohistochemistry
  • Hypoxia
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Arcasoy, M. O., Amin, K., Chou, S.-C., Haroon, Z. A., Varia, M., & Raleigh, J. A. (2005). Erythropoietin and erythropoietin receptor expression in head and neck cancer: relationship to tumor hypoxia. Clin Cancer Res, 11(1), 20–27.
Arcasoy, Murat O., Khalid Amin, Shu-Chuan Chou, Zishan A. Haroon, Mahesh Varia, and James A. Raleigh. “Erythropoietin and erythropoietin receptor expression in head and neck cancer: relationship to tumor hypoxia.Clin Cancer Res 11, no. 1 (January 1, 2005): 20–27.
Arcasoy MO, Amin K, Chou S-C, Haroon ZA, Varia M, Raleigh JA. Erythropoietin and erythropoietin receptor expression in head and neck cancer: relationship to tumor hypoxia. Clin Cancer Res. 2005 Jan 1;11(1):20–7.
Arcasoy, Murat O., et al. “Erythropoietin and erythropoietin receptor expression in head and neck cancer: relationship to tumor hypoxia.Clin Cancer Res, vol. 11, no. 1, Jan. 2005, pp. 20–27.
Arcasoy MO, Amin K, Chou S-C, Haroon ZA, Varia M, Raleigh JA. Erythropoietin and erythropoietin receptor expression in head and neck cancer: relationship to tumor hypoxia. Clin Cancer Res. 2005 Jan 1;11(1):20–27.

Published In

Clin Cancer Res

ISSN

1078-0432

Publication Date

January 1, 2005

Volume

11

Issue

1

Start / End Page

20 / 27

Location

United States

Related Subject Headings

  • Time Factors
  • Recombinant Proteins
  • Receptors, Erythropoietin
  • Radiation-Sensitizing Agents
  • Prognosis
  • Oncology & Carcinogenesis
  • Nitroimidazoles
  • Ligands
  • Immunohistochemistry
  • Hypoxia