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Transforming growth factor beta3 promotes fascial wound healing in a new animal model.

Publication ,  Journal Article
Tyrone, JW; Marcus, JR; Bonomo, SR; Mogford, JE; Xia, Y; Mustoe, TA
Published in: Arch Surg
October 2000

HYPOTHESIS: Transforming growth factor beta(3) (TGF-beta(3)) promotes fascial wound healing in a new animal model, as measured by wound breaking strength, collagen deposition, and cellular proliferation. DESIGN/INTERVENTION: Bilateral, longitudinal incisions were made in the anterior rectus sheaths of 24 male New Zealand white rabbits. One incision was treated with 1 microg of TGF-beta(3); the contralateral incision served as a control. The wounds were harvested at 1, 2, 3, 4, 6, and 8 weeks after creation ("wounding"). MAIN OUTCOME MEASURES: Wound tissue was tested for breaking strength using a tensiometer and processed for histological examination of collagen deposition and cellular proliferation at all time points after wounding. Collagen deposition and cellular proliferation were measured in histological cross sections of wounds with Masson trichrome staining and proliferating cell nuclear antigen immunohistochemistry, respectively. RESULTS: At all time points after wounding, treatment with TGF-beta(3) significantly increased the wound breaking strength (up to 138%) and collagen deposition (up to 150%) over the control group. Cellular proliferation was increased during the first 3 weeks after wounding (up to 147%), but returned to baseline levels by the fourth week. CONCLUSIONS: Transforming growth factor beta(3) promotes fascial wound healing. In this new animal model of fascial wound healing, TGF-beta(3) increased fascia breaking strength, collagen deposition, and cellular proliferation. These results are similar to findings in cutaneous wound models and demonstrate, for the first time, a pharmacologic agent to accelerate fascial healing.

Duke Scholars

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Published In

Arch Surg

DOI

ISSN

0004-0010

Publication Date

October 2000

Volume

135

Issue

10

Start / End Page

1154 / 1159

Location

United States

Related Subject Headings

  • Wounds and Injuries
  • Wound Healing
  • Transforming Growth Factor beta
  • Tensile Strength
  • Surgery
  • Reference Values
  • Rabbits
  • Muscle, Skeletal
  • Male
  • Intercellular Signaling Peptides and Proteins
 

Citation

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Tyrone, J. W., Marcus, J. R., Bonomo, S. R., Mogford, J. E., Xia, Y., & Mustoe, T. A. (2000). Transforming growth factor beta3 promotes fascial wound healing in a new animal model. Arch Surg, 135(10), 1154–1159. https://doi.org/10.1001/archsurg.135.10.1154
Tyrone, J. W., J. R. Marcus, S. R. Bonomo, J. E. Mogford, Y. Xia, and T. A. Mustoe. “Transforming growth factor beta3 promotes fascial wound healing in a new animal model.Arch Surg 135, no. 10 (October 2000): 1154–59. https://doi.org/10.1001/archsurg.135.10.1154.
Tyrone JW, Marcus JR, Bonomo SR, Mogford JE, Xia Y, Mustoe TA. Transforming growth factor beta3 promotes fascial wound healing in a new animal model. Arch Surg. 2000 Oct;135(10):1154–9.
Tyrone, J. W., et al. “Transforming growth factor beta3 promotes fascial wound healing in a new animal model.Arch Surg, vol. 135, no. 10, Oct. 2000, pp. 1154–59. Pubmed, doi:10.1001/archsurg.135.10.1154.
Tyrone JW, Marcus JR, Bonomo SR, Mogford JE, Xia Y, Mustoe TA. Transforming growth factor beta3 promotes fascial wound healing in a new animal model. Arch Surg. 2000 Oct;135(10):1154–1159.

Published In

Arch Surg

DOI

ISSN

0004-0010

Publication Date

October 2000

Volume

135

Issue

10

Start / End Page

1154 / 1159

Location

United States

Related Subject Headings

  • Wounds and Injuries
  • Wound Healing
  • Transforming Growth Factor beta
  • Tensile Strength
  • Surgery
  • Reference Values
  • Rabbits
  • Muscle, Skeletal
  • Male
  • Intercellular Signaling Peptides and Proteins