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A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.

Publication ,  Journal Article
Pei, ZM; Ward, JM; Harper, JF; Schroeder, JI
Published in: The EMBO journal
December 1996

Calcium-Dependent Protein Kinases (CDPKs) in higher plants contain a C-terminal calmodulin-like regulatory domain. Little is known regarding physiological CDPK targets. Both kinase activity and multiple Ca2+-dependent signaling pathways have been implicated in the control of stomatal guard cell movements. To determine whether CDPK or other protein kinases could have a role in guard cell signaling, purified and recombinant kinases were applied to Vicia faba guard cell vacuoles during patch-clamp experiments. CDPK activated novel vacuolar chloride (VCL) and malate conductances in guard cells. Activation was dependent on both Ca2+ and ATP. Furthermore, VCL activation occurred in the absence of Ca2+ using a Ca2+-independent, constitutively active, CDPK* mutant. Protein kinase A showed weaker activation (22% as compared with CDPK). Current reversals in whole vacuole recordings shifted with the Nernst potential for Cl-and vanished in glutamate. Single channel recordings showed a CDPK-activated 34 +/- 5 pS Cl- channel. VCL channels were activated at physiological potentials enabling Cl- uptake into vacuoles. VCL channels may provide a previously unidentified, but necessary, pathway for anion uptake into vacuoles required for stomatal opening. CDPK-activated VCL currents were also observed in red beet vacuoles suggesting that these channels may provide a more general mechanism for kinase-dependent anion uptake.

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Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

December 1996

Volume

15

Issue

23

Start / End Page

6564 / 6574

Related Subject Headings

  • Vegetables
  • Vacuoles
  • Species Specificity
  • Protein Serine-Threonine Kinases
  • Plants, Medicinal
  • Patch-Clamp Techniques
  • Membrane Potentials
  • Kinetics
  • Fabaceae
  • Developmental Biology
 

Citation

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Pei, Z. M., Ward, J. M., Harper, J. F., & Schroeder, J. I. (1996). A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK. The EMBO Journal, 15(23), 6564–6574. https://doi.org/10.1002/j.1460-2075.1996.tb01047.x
Pei, Z. M., J. M. Ward, J. F. Harper, and J. I. Schroeder. “A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.The EMBO Journal 15, no. 23 (December 1996): 6564–74. https://doi.org/10.1002/j.1460-2075.1996.tb01047.x.
Pei ZM, Ward JM, Harper JF, Schroeder JI. A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK. The EMBO journal. 1996 Dec;15(23):6564–74.
Pei, Z. M., et al. “A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK.The EMBO Journal, vol. 15, no. 23, Dec. 1996, pp. 6564–74. Epmc, doi:10.1002/j.1460-2075.1996.tb01047.x.
Pei ZM, Ward JM, Harper JF, Schroeder JI. A novel chloride channel in Vicia faba guard cell vacuoles activated by the serine/threonine kinase, CDPK. The EMBO journal. 1996 Dec;15(23):6564–6574.

Published In

The EMBO journal

DOI

EISSN

1460-2075

ISSN

0261-4189

Publication Date

December 1996

Volume

15

Issue

23

Start / End Page

6564 / 6574

Related Subject Headings

  • Vegetables
  • Vacuoles
  • Species Specificity
  • Protein Serine-Threonine Kinases
  • Plants, Medicinal
  • Patch-Clamp Techniques
  • Membrane Potentials
  • Kinetics
  • Fabaceae
  • Developmental Biology