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In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins.

Publication ,  Journal Article
Sampson, JH; Raman, A; Karlsen, G; Navsaria, H; Leigh, IM
Published in: Phytomedicine
May 2001

Psoriasis is a hyperproliferative skin disorder estimated to be present in 1-3% of most populations. Conventional therapy using corticosteroids, Vitamin D analogs and cytotoxic agents eg psoralens is associated with low success rate and many side effects. Traditional plant remedies may provide leads for new treatments. A rapid-throughput, in vitro bioassay has been utilised to examine plants for inhibitory effects on the growth of SVK-14 keratinocytes. Centella asiatica, a reputed anti-psoriatic herb, has been compared against the psoralen-containing seeds of Psoralea corylifolia and the synthetic anti-psoriatic agent dithranol (anthralin). Aqueous extracts of Psoralea corylifolia and Centella asiatica inhibited keratinocyte replication with IC50 values of 18.4 +/- 0.6 microg/ml and 209.9 +/- 9.8 mg/ml respectively prior to treatment with polyvinylpolypyrrolidone (PVPP) and 36.3 +/- 3.3 mg/ml and 238.0 +/- 2.5 mg/ml respectively after PVPP treatment of the extracts. The effect produced by C. asiatica is thus unlikely to be due to phenolic compounds. It may, however, be due to its two constituent triterpenoid glycosides madecassoside and asiaticoside which had IC50 values of 8.6 +/- 0.6 microM respectively. These values were comparable to their concentrations in the crude extract and to the IC50 of dithranol (5.1 +/- 0.4 microM). These results suggest that the potential use of C. asiatica extracts as a topical anti-psoriatic agent is worthy of further investigation.

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Published In

Phytomedicine

DOI

ISSN

0944-7113

Publication Date

May 2001

Volume

8

Issue

3

Start / End Page

230 / 235

Location

Germany

Related Subject Headings

  • Tumor Cells, Cultured
  • Triterpenes
  • Titrimetry
  • Saponins
  • Rhodamines
  • Psoriasis
  • Plants, Medicinal
  • Plant Extracts
  • Phytotherapy
  • Medicinal & Biomolecular Chemistry
 

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Sampson, J. H., Raman, A., Karlsen, G., Navsaria, H., & Leigh, I. M. (2001). In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins. Phytomedicine, 8(3), 230–235. https://doi.org/10.1078/0944-7113-00032
Sampson, J. H., A. Raman, G. Karlsen, H. Navsaria, and I. M. Leigh. “In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins.Phytomedicine 8, no. 3 (May 2001): 230–35. https://doi.org/10.1078/0944-7113-00032.
Sampson JH, Raman A, Karlsen G, Navsaria H, Leigh IM. In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins. Phytomedicine. 2001 May;8(3):230–5.
Sampson, J. H., et al. “In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins.Phytomedicine, vol. 8, no. 3, May 2001, pp. 230–35. Pubmed, doi:10.1078/0944-7113-00032.
Sampson JH, Raman A, Karlsen G, Navsaria H, Leigh IM. In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins. Phytomedicine. 2001 May;8(3):230–235.
Journal cover image

Published In

Phytomedicine

DOI

ISSN

0944-7113

Publication Date

May 2001

Volume

8

Issue

3

Start / End Page

230 / 235

Location

Germany

Related Subject Headings

  • Tumor Cells, Cultured
  • Triterpenes
  • Titrimetry
  • Saponins
  • Rhodamines
  • Psoriasis
  • Plants, Medicinal
  • Plant Extracts
  • Phytotherapy
  • Medicinal & Biomolecular Chemistry