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The effect of exposure time on microsurgical anastomoses of experimentally crushed arteries.

Publication ,  Journal Article
Su, WF; Chen, LE; Seaber, AV; Urbaniak, JR
Published in: Int Angiol
September 1995

Replantation after crushing amputation has a relatively low success rate. Although the mechanism of trauma is a major factor in failure, the time lapse before vessel anastomosis may also be a contributing factor. In this study, we observed the influence of the interval between vessel injury and surgical treatment on thrombus formation and healing after controlled crushing. Seventy-five Sprague-Dawley rats were used. A segment of femoral artery was clamped to create warm ischemia for 8 hours and crushed with a 15 kg load for one hour. After the loading device was removed the crushed segments were transected and the vessel ends exposed to the adjacent tissues and blood for 0, 2, 4 and 6 hours (groups II-V, respectively) prior to being anastomosed with standard microsurgical technique. The vessel samples were harvested at days 1, 2 and 7, respectively, and evaluated by light microscopy and scanning electron microscopy (SEM). The patency rate of the anastomoses was 97.3% at harvest and reendothelialization was completed at day 7. Three anastomoses with 4 or 6 hours exposure showed thrombosis, or clotting. The results indicated that up to 6 hours exposure time did not have a significant influence on thrombus formation or the healing process of vessels under the controlled conditions of this study.

Duke Scholars

Published In

Int Angiol

ISSN

0392-9590

Publication Date

September 1995

Volume

14

Issue

3

Start / End Page

243 / 247

Location

Italy

Related Subject Headings

  • Wound Healing
  • Thrombosis
  • Replantation
  • Rats, Sprague-Dawley
  • Rats
  • Muscle, Smooth, Vascular
  • Microsurgery
  • Microscopy, Electron, Scanning
  • Ischemia
  • Hindlimb
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Su, W. F., Chen, L. E., Seaber, A. V., & Urbaniak, J. R. (1995). The effect of exposure time on microsurgical anastomoses of experimentally crushed arteries. Int Angiol, 14(3), 243–247.
Su, W. F., L. E. Chen, A. V. Seaber, and J. R. Urbaniak. “The effect of exposure time on microsurgical anastomoses of experimentally crushed arteries.Int Angiol 14, no. 3 (September 1995): 243–47.
Su WF, Chen LE, Seaber AV, Urbaniak JR. The effect of exposure time on microsurgical anastomoses of experimentally crushed arteries. Int Angiol. 1995 Sep;14(3):243–7.
Su, W. F., et al. “The effect of exposure time on microsurgical anastomoses of experimentally crushed arteries.Int Angiol, vol. 14, no. 3, Sept. 1995, pp. 243–47.
Su WF, Chen LE, Seaber AV, Urbaniak JR. The effect of exposure time on microsurgical anastomoses of experimentally crushed arteries. Int Angiol. 1995 Sep;14(3):243–247.

Published In

Int Angiol

ISSN

0392-9590

Publication Date

September 1995

Volume

14

Issue

3

Start / End Page

243 / 247

Location

Italy

Related Subject Headings

  • Wound Healing
  • Thrombosis
  • Replantation
  • Rats, Sprague-Dawley
  • Rats
  • Muscle, Smooth, Vascular
  • Microsurgery
  • Microscopy, Electron, Scanning
  • Ischemia
  • Hindlimb