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NF-kappaB p65 involves in reperfusion injury and iNOS gene regulation in skeletal muscle.

Publication ,  Journal Article
Qi, W-N; Chaiyakit, P; Cai, Y; Allen, DM; Chen, L-E; Seaber, AV; Urbaniak, JR
Published in: Microsurgery
2004

This study investigated the effects of inhibition of NF-kappaB activation on microcirculation and inducible NOS expression in reperfused rat cremaster muscle. The muscle from 16 rats underwent 5-h ischemia and 90-min reperfusion. Each rat received NF-kappaB inhibitor pyrrolidine dithiocarbamate (PDTC, 150 mg/kg) or phosphate-buffered saline 15 min before reperfusion. Results showed that PDTC treatment had a significant overall increase in muscle blood flow during reperfusion. Blood flow more rapidly recovered to and over baseline in the PDTC-treated group than in controls, with a significant difference at 10-30 min and 70-90 min. Expression of iNOS mRNA had a 167-fold increase from normal in controls, but was significantly (P < 0.05) reduced to a 63-fold increase in PDTC-treated muscles. In addition, PDTC treatment significantly (P < 0.05) decreased a reperfusion-induced increase in activated NF-kappaB p65 and nuclear p65 protein. Our results suggest that NF-kappaB is involved in I/R injury and that inhibition of NF-kappaB p65 activation affords protection against I/R injury, perhaps via downregulating expression of iNOS transcription.

Duke Scholars

Published In

Microsurgery

DOI

ISSN

0738-1085

Publication Date

2004

Volume

24

Issue

4

Start / End Page

316 / 323

Location

United States

Related Subject Headings

  • Transcription Factor RelA
  • Thiocarbamates
  • Surgery
  • Reperfusion Injury
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Pyrrolidines
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase
 

Citation

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Chicago
ICMJE
MLA
NLM
Qi, W.-N., Chaiyakit, P., Cai, Y., Allen, D. M., Chen, L.-E., Seaber, A. V., & Urbaniak, J. R. (2004). NF-kappaB p65 involves in reperfusion injury and iNOS gene regulation in skeletal muscle. Microsurgery, 24(4), 316–323. https://doi.org/10.1002/micr.20030
Qi, Wen-Ning, Pruk Chaiyakit, Yongting Cai, Diane M. Allen, Long-En Chen, Anthony V. Seaber, and James R. Urbaniak. “NF-kappaB p65 involves in reperfusion injury and iNOS gene regulation in skeletal muscle.Microsurgery 24, no. 4 (2004): 316–23. https://doi.org/10.1002/micr.20030.
Qi W-N, Chaiyakit P, Cai Y, Allen DM, Chen L-E, Seaber AV, et al. NF-kappaB p65 involves in reperfusion injury and iNOS gene regulation in skeletal muscle. Microsurgery. 2004;24(4):316–23.
Qi, Wen-Ning, et al. “NF-kappaB p65 involves in reperfusion injury and iNOS gene regulation in skeletal muscle.Microsurgery, vol. 24, no. 4, 2004, pp. 316–23. Pubmed, doi:10.1002/micr.20030.
Qi W-N, Chaiyakit P, Cai Y, Allen DM, Chen L-E, Seaber AV, Urbaniak JR. NF-kappaB p65 involves in reperfusion injury and iNOS gene regulation in skeletal muscle. Microsurgery. 2004;24(4):316–323.
Journal cover image

Published In

Microsurgery

DOI

ISSN

0738-1085

Publication Date

2004

Volume

24

Issue

4

Start / End Page

316 / 323

Location

United States

Related Subject Headings

  • Transcription Factor RelA
  • Thiocarbamates
  • Surgery
  • Reperfusion Injury
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Pyrrolidines
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Synthase