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Adjacent tissues (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro.

Publication ,  Journal Article
Tognana, E; Chen, F; Padera, RF; Leddy, HA; Christensen, SE; Guilak, F; Vunjak-Novakovic, G; Freed, LE
Published in: Osteoarthritis and cartilage
February 2005

An in vitro model was used to test the hypothesis that culture time and adjacent tissue structure and composition affected chondrogenesis and integrative repair in engineered cartilage.Engineered constructs made of bovine calf chondrocytes and hyaluronan benzyl ester non-woven mesh were press-fitted into adjacent tissue rings made of articular cartilage (AC), devitalized bone (DB), or vital bone (VB) and cultured in rotating bioreactors for up to 8 weeks. Structure (light and electron microscopy), biomechanical properties (interfacial adhesive strength, construct compressive modulus), biochemical composition (construct glycosaminoglycans (GAG), collagen, and cells), and adjacent tissue diffusivity were assessed.Engineered constructs were comprised predominately of hyaline cartilage, and appeared either closely apposed to adjacent cartilage or functionally interdigitated with adjacent bone due to interfacial deposition of extracellular matrix. An increase in culture time significantly improved construct adhesive strength (P<0.001), modulus (P=0.02), GAG (P=0.04) and cellularity (P<0.001). The type of adjacent tissue significantly affected construct adhesion (P<0.001), modulus (P<0.001), GAG (P<0.001) and collagen (P<0.001). For constructs cultured in rings of cartilage, negative correlations were observed between ring GAG content (log transformed) and construct adhesion (R2=0.66, P<0.005), modulus (R2=0.49, P<0.05) and GAG (R2=0.44, P<0.05). Integrative repair was better for constructs cultured adjacent to bone than cartilage, in association with its solid architectural structure and high GAG content, and best for constructs cultured adjacent to DB, in association with its high diffusivity.Chondrogenesis and integrative repair in engineered cartilage improved with time and depended on adjacent tissue architecture, composition, and transport properties.

Duke Scholars

Published In

Osteoarthritis and cartilage

DOI

EISSN

1522-9653

ISSN

1063-4584

Publication Date

February 2005

Volume

13

Issue

2

Start / End Page

129 / 138

Related Subject Headings

  • Tissue Engineering
  • Time Factors
  • Microscopy, Electron
  • Hyaluronic Acid
  • Fibrillar Collagens
  • Diffusion
  • Chondrogenesis
  • Chondrocytes
  • Cells, Cultured
  • Cattle
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tognana, E., Chen, F., Padera, R. F., Leddy, H. A., Christensen, S. E., Guilak, F., … Freed, L. E. (2005). Adjacent tissues (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro. Osteoarthritis and Cartilage, 13(2), 129–138. https://doi.org/10.1016/j.joca.2004.10.015
Tognana, E., F. Chen, R. F. Padera, H. A. Leddy, S. E. Christensen, F. Guilak, G. Vunjak-Novakovic, and L. E. Freed. “Adjacent tissues (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro.Osteoarthritis and Cartilage 13, no. 2 (February 2005): 129–38. https://doi.org/10.1016/j.joca.2004.10.015.
Tognana E, Chen F, Padera RF, Leddy HA, Christensen SE, Guilak F, et al. Adjacent tissues (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro. Osteoarthritis and cartilage. 2005 Feb;13(2):129–38.
Tognana, E., et al. “Adjacent tissues (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro.Osteoarthritis and Cartilage, vol. 13, no. 2, Feb. 2005, pp. 129–38. Epmc, doi:10.1016/j.joca.2004.10.015.
Tognana E, Chen F, Padera RF, Leddy HA, Christensen SE, Guilak F, Vunjak-Novakovic G, Freed LE. Adjacent tissues (cartilage, bone) affect the functional integration of engineered calf cartilage in vitro. Osteoarthritis and cartilage. 2005 Feb;13(2):129–138.
Journal cover image

Published In

Osteoarthritis and cartilage

DOI

EISSN

1522-9653

ISSN

1063-4584

Publication Date

February 2005

Volume

13

Issue

2

Start / End Page

129 / 138

Related Subject Headings

  • Tissue Engineering
  • Time Factors
  • Microscopy, Electron
  • Hyaluronic Acid
  • Fibrillar Collagens
  • Diffusion
  • Chondrogenesis
  • Chondrocytes
  • Cells, Cultured
  • Cattle