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Isolation of a nuclease-resistant decoy RNA that can protect human acetylcholine receptors from myasthenic antibodies.

Publication ,  Journal Article
Lee, SW; Sullenger, BA
Published in: Nat Biotechnol
January 1997

The muscular weakness and fatigability associated with myasthenia gravis are engendered by autoantibodies directed against acetylcholine receptors on muscle cells at neuromuscular junctions. The pathogenic consequences of this immune response can potentially be modulated by molecules that bind such autoantibodies and block their interaction with these receptors. We report the isolation of a small nuclease-resistant RNA molecule that binds both a rat monoclonal antibody that recognizes the main immunogenic region on the acetylcholine receptor, and autoantibodies from patients with myasthenia gravis. Moreover, this RNA can act as a decoy and protect acetylcholine receptors on human cells from the effects of these antibodies.

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Published In

Nat Biotechnol

DOI

ISSN

1087-0156

Publication Date

January 1997

Volume

15

Issue

1

Start / End Page

41 / 45

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Substrate Specificity
  • Ribonucleases
  • Rhabdomyosarcoma
  • Receptors, Cholinergic
  • Rats
  • RNA
  • Myasthenia Gravis
  • Molecular Sequence Data
  • Humans
 

Citation

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Lee, S. W., & Sullenger, B. A. (1997). Isolation of a nuclease-resistant decoy RNA that can protect human acetylcholine receptors from myasthenic antibodies. Nat Biotechnol, 15(1), 41–45. https://doi.org/10.1038/nbt0197-41
Lee, S. W., and B. A. Sullenger. “Isolation of a nuclease-resistant decoy RNA that can protect human acetylcholine receptors from myasthenic antibodies.Nat Biotechnol 15, no. 1 (January 1997): 41–45. https://doi.org/10.1038/nbt0197-41.
Lee, S. W., and B. A. Sullenger. “Isolation of a nuclease-resistant decoy RNA that can protect human acetylcholine receptors from myasthenic antibodies.Nat Biotechnol, vol. 15, no. 1, Jan. 1997, pp. 41–45. Pubmed, doi:10.1038/nbt0197-41.

Published In

Nat Biotechnol

DOI

ISSN

1087-0156

Publication Date

January 1997

Volume

15

Issue

1

Start / End Page

41 / 45

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Substrate Specificity
  • Ribonucleases
  • Rhabdomyosarcoma
  • Receptors, Cholinergic
  • Rats
  • RNA
  • Myasthenia Gravis
  • Molecular Sequence Data
  • Humans