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Tissue culture of epithelium derived from Barrett's oesophagus.

Publication ,  Journal Article
Washington, K; Gottfried, MR; Telen, MJ
Published in: Gut
July 1994

Barrett's oesophagus is a preneoplastic condition in which the squamous mucosa of the oesophagus is replaced by columnar epithelium. Epithelial cells of Barrett's oesophagus were isolated from resected oesophagus specimens by two methods not previously applied to the culture of Barrett's oesophagus cells. These techniques included trypsinisation of small fragments of mucosa, followed by plating in tissue culture dishes, and a direct tissue explant technique. A modified MCDB-153 growth medium was used. Primary trypsin technique cultures were plated on uncoated plastic, or plastic coated with type I collagen, type IV collagen, or fibronectin. Growth on type IV collagen and fibronectin plates was slower but produced less contamination from fibroblasts. By 20-40 days most cultures formed confluent monolayers made up of cells with epithelial morphology. The cells were cytokeratin positive, vimentin negative, and contained alcian blue positive vacuoles, confirming their epithelial origin and suggesting their derivation from Barrett's oesophagus. Electron microscopy showed tonofilaments, microvilli, and desmosomes. Cells proliferated through up to eight subcultures before growth slowed and cells showed senescent changes. This study shows that epithelial cells from Barrett's oesophagus can be grown by comparatively simple tissue culture techniques. These methods can provide sufficient material for a variety of molecular biology and biochemical studies of epithelial cells from Barrett's oesophagus.

Duke Scholars

Published In

Gut

DOI

ISSN

0017-5749

Publication Date

July 1994

Volume

35

Issue

7

Start / End Page

879 / 883

Location

England

Related Subject Headings

  • Microscopy, Electron
  • Humans
  • Gastroenterology & Hepatology
  • Esophagus
  • Epithelium
  • Culture Techniques
  • Culture Media
  • Barrett Esophagus
  • 3210 Nutrition and dietetics
  • 3202 Clinical sciences
 

Citation

APA
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ICMJE
MLA
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Washington, K., Gottfried, M. R., & Telen, M. J. (1994). Tissue culture of epithelium derived from Barrett's oesophagus. Gut, 35(7), 879–883. https://doi.org/10.1136/gut.35.7.879
Washington, K., M. R. Gottfried, and M. J. Telen. “Tissue culture of epithelium derived from Barrett's oesophagus.Gut 35, no. 7 (July 1994): 879–83. https://doi.org/10.1136/gut.35.7.879.
Washington K, Gottfried MR, Telen MJ. Tissue culture of epithelium derived from Barrett's oesophagus. Gut. 1994 Jul;35(7):879–83.
Washington, K., et al. “Tissue culture of epithelium derived from Barrett's oesophagus.Gut, vol. 35, no. 7, July 1994, pp. 879–83. Pubmed, doi:10.1136/gut.35.7.879.
Washington K, Gottfried MR, Telen MJ. Tissue culture of epithelium derived from Barrett's oesophagus. Gut. 1994 Jul;35(7):879–883.

Published In

Gut

DOI

ISSN

0017-5749

Publication Date

July 1994

Volume

35

Issue

7

Start / End Page

879 / 883

Location

England

Related Subject Headings

  • Microscopy, Electron
  • Humans
  • Gastroenterology & Hepatology
  • Esophagus
  • Epithelium
  • Culture Techniques
  • Culture Media
  • Barrett Esophagus
  • 3210 Nutrition and dietetics
  • 3202 Clinical sciences