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Leukocyte phenotypic changes in an in vitro model of ABO hemolytic transfusion reaction.

Publication ,  Journal Article
Udani, M; Rao, N; Telen, MJ
Published in: Transfusion
September 1997

BACKGROUND: ABO antigen-antibody interaction in the presence of peripheral blood leukocytes (white cells) results in the production of a variety of proinflammatory cytokines. However, although tumor necrosis factor alpha has been shown to be derived at least primarily from monocytes, the range of cells activated by this process has not previously been reported. Therefore, changes in mononuclear cell surface antigen expression were studied, to determine which subsets of white cells appeared to be activated in the setting of ABO incompatibility. STUDY DESIGN AND METHODS: Group O peripheral blood mononuclear cells (PBMCs) were incubated in autologous plasma with group A or O red cells (RBCs) for up to 24 hours. White cell expression of activation and adhesion markers was measured at 2 and 24 hours by flow cytometry, using direct or indirect fluorescein or phycoerythrin labeling. RESULTS: Expression of lymphocyte activation markers CD25, CDw108, and CD109 was equivalent when PBMCs incubated with group A and O RBCs were compared. However, after 2 hours, mean fluorescence of CD14 on PBMCs incubated with group A RBCs was 65 percent of that on PBMCs incubated with group O RBCs and remained similarly decreased at 24 hours. CD44 expression was upregulated on PBMCs exposed to both group A and O RBCs, but it was increased significantly more on monocytes exposed to group A RBCs. The ability to bind hyaluronic acid was induced in approximately 42 percent of CD14+ monocytes exposed to group A RBCs but in no cells exposed to group O RBCs. CONCLUSION: Downregulation of CD14 and increased binding of hyaluronic acid reflects monocyte activation in this model. No evidence of lymphocyte activation was found, supporting the hypothesis that ABO transfusion reactions primarily activate monocytes.

Duke Scholars

Published In

Transfusion

DOI

ISSN

0041-1132

Publication Date

September 1997

Volume

37

Issue

9

Start / End Page

904 / 909

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Transfusion Reaction
  • Phenotype
  • Lymphocyte Activation
  • Leukocytes
  • Hyaluronan Receptors
  • Humans
  • Hemolysis
  • Hemoglobins
  • Fluorescent Antibody Technique, Indirect
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Udani, M., Rao, N., & Telen, M. J. (1997). Leukocyte phenotypic changes in an in vitro model of ABO hemolytic transfusion reaction. Transfusion, 37(9), 904–909. https://doi.org/10.1046/j.1537-2995.1997.37997454015.x
Udani, M., N. Rao, and M. J. Telen. “Leukocyte phenotypic changes in an in vitro model of ABO hemolytic transfusion reaction.Transfusion 37, no. 9 (September 1997): 904–9. https://doi.org/10.1046/j.1537-2995.1997.37997454015.x.
Udani M, Rao N, Telen MJ. Leukocyte phenotypic changes in an in vitro model of ABO hemolytic transfusion reaction. Transfusion. 1997 Sep;37(9):904–9.
Udani, M., et al. “Leukocyte phenotypic changes in an in vitro model of ABO hemolytic transfusion reaction.Transfusion, vol. 37, no. 9, Sept. 1997, pp. 904–09. Pubmed, doi:10.1046/j.1537-2995.1997.37997454015.x.
Udani M, Rao N, Telen MJ. Leukocyte phenotypic changes in an in vitro model of ABO hemolytic transfusion reaction. Transfusion. 1997 Sep;37(9):904–909.
Journal cover image

Published In

Transfusion

DOI

ISSN

0041-1132

Publication Date

September 1997

Volume

37

Issue

9

Start / End Page

904 / 909

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Transfusion Reaction
  • Phenotype
  • Lymphocyte Activation
  • Leukocytes
  • Hyaluronan Receptors
  • Humans
  • Hemolysis
  • Hemoglobins
  • Fluorescent Antibody Technique, Indirect