Skip to main content
Journal cover image

The inhibition of human adrenal steroidogenic enzyme activities by suramin.

Publication ,  Journal Article
Ashby, H; DiMattina, M; Linehan, WM; Robertson, CN; Queenan, JT; Albertson, BD
Published in: J Clin Endocrinol Metab
February 1989

Suramin has recently been used to treat patients with acquired immune deficiency syndrome because of the action of this drug on reverse transcriptase. Patients so treated developed the symptoms and hormonal profiles of adrenal insufficiency. To evaluate the mechanism of action of suramin on adrenalcortical function, adrenal mitochondrial and microsomal preparations from five subjects were assayed for steroidogenic enzyme activity in the presence and absence of suramin. Specifically, 3 beta-hydroxysteroid dehydrogenase/isomerase, 17 alpha-hydroxylase, 21-hydroxylase, 11 beta-hydroxylase, and 17,20-desmolase activities were measured in the presence of 0-5000 mumol/L suramin concentrations. In all assays, enzyme activities decreased in a dose-dependent fashion as suramin concentrations increased. The drug doses (calculated) that caused 50% inhibition of enzyme activity were: 21-hydroxylase activity, 50 mumol/L; 17 alpha-hydroxylase activity, 25 mumol/L; 17,20-desmolase activity, 50 mumol/L; 11 beta-hydroxylase, 2 mumol/L, and 3 beta-hydroxysteroid dehydrogenase/isomerase, 1200 mumol/L. These results suggest that suramin has a concentration-dependent inhibitory effect on the key P-450-regulated enzymatic steps in adrenal glucocorticoid steroidogenesis, which may explain the development of adrenal insufficiency in acquired immune deficiency syndrome patients treated with suramin.

Duke Scholars

Published In

J Clin Endocrinol Metab

DOI

ISSN

0021-972X

Publication Date

February 1989

Volume

68

Issue

2

Start / End Page

505 / 508

Location

United States

Related Subject Headings

  • Suramin
  • Steroid Isomerases
  • Steroid Hydroxylases
  • Reverse Transcriptase Inhibitors
  • Mitochondria
  • Microsomes
  • Hydroxysteroid Dehydrogenases
  • Humans
  • Endocrinology & Metabolism
  • Adrenal Cortex
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ashby, H., DiMattina, M., Linehan, W. M., Robertson, C. N., Queenan, J. T., & Albertson, B. D. (1989). The inhibition of human adrenal steroidogenic enzyme activities by suramin. J Clin Endocrinol Metab, 68(2), 505–508. https://doi.org/10.1210/jcem-68-2-505
Ashby, H., M. DiMattina, W. M. Linehan, C. N. Robertson, J. T. Queenan, and B. D. Albertson. “The inhibition of human adrenal steroidogenic enzyme activities by suramin.J Clin Endocrinol Metab 68, no. 2 (February 1989): 505–8. https://doi.org/10.1210/jcem-68-2-505.
Ashby H, DiMattina M, Linehan WM, Robertson CN, Queenan JT, Albertson BD. The inhibition of human adrenal steroidogenic enzyme activities by suramin. J Clin Endocrinol Metab. 1989 Feb;68(2):505–8.
Ashby, H., et al. “The inhibition of human adrenal steroidogenic enzyme activities by suramin.J Clin Endocrinol Metab, vol. 68, no. 2, Feb. 1989, pp. 505–08. Pubmed, doi:10.1210/jcem-68-2-505.
Ashby H, DiMattina M, Linehan WM, Robertson CN, Queenan JT, Albertson BD. The inhibition of human adrenal steroidogenic enzyme activities by suramin. J Clin Endocrinol Metab. 1989 Feb;68(2):505–508.
Journal cover image

Published In

J Clin Endocrinol Metab

DOI

ISSN

0021-972X

Publication Date

February 1989

Volume

68

Issue

2

Start / End Page

505 / 508

Location

United States

Related Subject Headings

  • Suramin
  • Steroid Isomerases
  • Steroid Hydroxylases
  • Reverse Transcriptase Inhibitors
  • Mitochondria
  • Microsomes
  • Hydroxysteroid Dehydrogenases
  • Humans
  • Endocrinology & Metabolism
  • Adrenal Cortex