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Studies of tumor-infiltrating lymphocytes from a human choroidal melanoma.

Publication ,  Journal Article
Ksander, BR; Rubsamen, PE; Olsen, KR; Cousins, SW; Streilein, JW
Published in: Invest Ophthalmol Vis Sci
December 1991

Cell suspensions prepared enzymatically from an ocular choroidal melanoma were cultured in vitro in an effort to generate (1) melanoma tumor cell lines and (2) tumor-infiltrating lymphocytes cytotoxic for ocular melanoma cells. Even though histologic study of the tumor did not show "significant" infiltrating bone marrow-derived cells, lymphocytes were generated readily in cultures to which interleukin-2 was added. Phenotypic analysis of the cultured lymphocytes indicated that T-cells, natural killer (NK) cells, and lymphokine-activated killer (LAK) cells were present. Moreover, functional studies of the cultured lymphocytes revealed NK activity, LAK activity, and most importantly, tumor antigen-specific cytotoxic T-cell activity. It was concluded that it is possible to obtain tumor cell lines and tumor-infiltrating lymphocytes from ocular tumors, both of which would be required if cellular immunotherapy of ocular tumors is contemplated. In addition, these results indicate that ocular melanomas can express unique tumor-specific antigens and that the immune system of a patient with such an ocular tumor can perceive these tumor antigens because antigen-specific precursor cytotoxic T-cells were present in the tumor-containing eye at the time of enucleation. The theoretic and therapeutic implications of these findings are discussed.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

December 1991

Volume

32

Issue

13

Start / End Page

3198 / 3208

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • T-Lymphocytes
  • Ophthalmology & Optometry
  • Melanoma
  • Lymphocytes, Tumor-Infiltrating
  • Immunophenotyping
  • Humans
  • Flow Cytometry
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ksander, B. R., Rubsamen, P. E., Olsen, K. R., Cousins, S. W., & Streilein, J. W. (1991). Studies of tumor-infiltrating lymphocytes from a human choroidal melanoma. Invest Ophthalmol Vis Sci, 32(13), 3198–3208.
Ksander, B. R., P. E. Rubsamen, K. R. Olsen, S. W. Cousins, and J. W. Streilein. “Studies of tumor-infiltrating lymphocytes from a human choroidal melanoma.Invest Ophthalmol Vis Sci 32, no. 13 (December 1991): 3198–3208.
Ksander BR, Rubsamen PE, Olsen KR, Cousins SW, Streilein JW. Studies of tumor-infiltrating lymphocytes from a human choroidal melanoma. Invest Ophthalmol Vis Sci. 1991 Dec;32(13):3198–208.
Ksander, B. R., et al. “Studies of tumor-infiltrating lymphocytes from a human choroidal melanoma.Invest Ophthalmol Vis Sci, vol. 32, no. 13, Dec. 1991, pp. 3198–208.
Ksander BR, Rubsamen PE, Olsen KR, Cousins SW, Streilein JW. Studies of tumor-infiltrating lymphocytes from a human choroidal melanoma. Invest Ophthalmol Vis Sci. 1991 Dec;32(13):3198–3208.

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

December 1991

Volume

32

Issue

13

Start / End Page

3198 / 3208

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • T-Lymphocytes
  • Ophthalmology & Optometry
  • Melanoma
  • Lymphocytes, Tumor-Infiltrating
  • Immunophenotyping
  • Humans
  • Flow Cytometry
  • Female