Deletion-mutant epidermal growth factor receptor in human gliomas: effects of type II mutation on receptor function.
Malignant human glioma D-298 MG amplifies a rearranged epidermal growth factor receptor (EGFR) gene (c-erbB proto-oncogene), resulting in an in-frame deletion of 83 amino acids in domain IV of the extracellular domain of the EGFR. EGF and transforming growth factor-a (TGF-a) bound to the mutant EGFR with high affinity and enhanced the intrinsic mutant EGFR kinase activity. The mutant EGFR was capable of transducing EGF-stimulated glioma cell proliferation and invasiveness in an in vitro three-dimensional spheroid model. The deletion-mutant EGFR in D-298 MG is capable of being activated by growth factor; this suggests that overexpression of this mutant EGFR protein rather than structural alteration may be the more significant biologic event.
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- Transforming Growth Factor alpha
- Receptor, erbB-2
- Receptor, ErbB-2
- Proto-Oncogenes
- Proto-Oncogene Proteins
- Proto-Oncogene Mas
- Protein-Tyrosine Kinases
- Phosphorylation
- Molecular Sequence Data
- Kinetics
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transforming Growth Factor alpha
- Receptor, erbB-2
- Receptor, ErbB-2
- Proto-Oncogenes
- Proto-Oncogene Proteins
- Proto-Oncogene Mas
- Protein-Tyrosine Kinases
- Phosphorylation
- Molecular Sequence Data
- Kinetics