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Improved techniques for staining and enumerating focus formation in viral infectivity assays.

Publication ,  Journal Article
Self, DJ; Pettiss, KF; Bigner, DD
Published in: Appl Microbiol
July 1974

Improved techniques consisting of glutaraldehyde fixation, Giemsa staining, and colony counter enumeration of foci in viral infectivity assays were compared to microscope scanning and lantern slide projection methods. These techniques broadened the scope of application to potentially hazardous viruses and increased the efficiency of assaying.

Duke Scholars

Published In

Appl Microbiol

DOI

ISSN

0003-6919

Publication Date

July 1974

Volume

28

Issue

1

Start / End Page

156 / 157

Location

United States

Related Subject Headings

  • Viruses
  • Viral Plaque Assay
  • Staining and Labeling
  • Microscopy
  • Methods
  • Glutaral
  • Coloring Agents
  • Chick Embryo
  • Cell Line
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Self, D. J., Pettiss, K. F., & Bigner, D. D. (1974). Improved techniques for staining and enumerating focus formation in viral infectivity assays. Appl Microbiol, 28(1), 156–157. https://doi.org/10.1128/am.28.1.156-157.1974
Self, D. J., K. F. Pettiss, and D. D. Bigner. “Improved techniques for staining and enumerating focus formation in viral infectivity assays.Appl Microbiol 28, no. 1 (July 1974): 156–57. https://doi.org/10.1128/am.28.1.156-157.1974.
Self DJ, Pettiss KF, Bigner DD. Improved techniques for staining and enumerating focus formation in viral infectivity assays. Appl Microbiol. 1974 Jul;28(1):156–7.
Self, D. J., et al. “Improved techniques for staining and enumerating focus formation in viral infectivity assays.Appl Microbiol, vol. 28, no. 1, July 1974, pp. 156–57. Pubmed, doi:10.1128/am.28.1.156-157.1974.
Self DJ, Pettiss KF, Bigner DD. Improved techniques for staining and enumerating focus formation in viral infectivity assays. Appl Microbiol. 1974 Jul;28(1):156–157.

Published In

Appl Microbiol

DOI

ISSN

0003-6919

Publication Date

July 1974

Volume

28

Issue

1

Start / End Page

156 / 157

Location

United States

Related Subject Headings

  • Viruses
  • Viral Plaque Assay
  • Staining and Labeling
  • Microscopy
  • Methods
  • Glutaral
  • Coloring Agents
  • Chick Embryo
  • Cell Line
  • Animals