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A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits.

Publication ,  Journal Article
Trojanowski, JQ; Friedman, HS; Burger, PC; Bigner, DD
Published in: Am J Pathol
February 1987

The majority of cells in a rapidly dividing human medulloblastoma cell line (D283 MED) are shown to express the two high-molecular-weight human neurofilament (NF) subunits, whereas a minority express the low-molecular-weight NF subunit. These three polypeptides are integral subunits of the intermediate filaments (IFs) found in normal neurons. Other cell type-specific IF proteins (keratin, desmin, and glial filament polypeptides) are not present in D283 MED cells. Further, the immunocytochemical, immunochemical and ultrastructural data suggest that the neurofilaments in these cells are abnormal, possibly because of a paucity of the low-molecular-weight NF subunit. This is the first human cell line derived from a central nervous system tumor that is capable of expressing all three NF triplet proteins. It is a unique model system for studies of normal and abnormal human NF metabolism as well as for probing the cell biology of medulloblastomas.

Duke Scholars

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

February 1987

Volume

126

Issue

2

Start / End Page

358 / 363

Location

United States

Related Subject Headings

  • Pathology
  • Neurofilament Proteins
  • Neoplasm Transplantation
  • Molecular Weight
  • Microscopy, Electron
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Intermediate Filament Proteins
  • Immunologic Techniques
 

Citation

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Trojanowski, J. Q., Friedman, H. S., Burger, P. C., & Bigner, D. D. (1987). A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits. Am J Pathol, 126(2), 358–363.
Trojanowski, J. Q., H. S. Friedman, P. C. Burger, and D. D. Bigner. “A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits.Am J Pathol 126, no. 2 (February 1987): 358–63.
Trojanowski JQ, Friedman HS, Burger PC, Bigner DD. A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits. Am J Pathol. 1987 Feb;126(2):358–63.
Trojanowski, J. Q., et al. “A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits.Am J Pathol, vol. 126, no. 2, Feb. 1987, pp. 358–63.
Trojanowski JQ, Friedman HS, Burger PC, Bigner DD. A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits. Am J Pathol. 1987 Feb;126(2):358–363.
Journal cover image

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

February 1987

Volume

126

Issue

2

Start / End Page

358 / 363

Location

United States

Related Subject Headings

  • Pathology
  • Neurofilament Proteins
  • Neoplasm Transplantation
  • Molecular Weight
  • Microscopy, Electron
  • Mice, Nude
  • Mice
  • Medulloblastoma
  • Intermediate Filament Proteins
  • Immunologic Techniques