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Tenascin expression in human glioma cell lines and normal tissues.

Publication ,  Journal Article
Ventimiglia, JB; Wikstrand, CJ; Ostrowski, LE; Bourdon, MA; Lightner, VA; Bigner, DD
Published in: J Neuroimmunol
January 1992
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Tenascin expression was evaluated in 21 human glioma cell lines and in normal adult tissue extracts by Western and Northern blotting. The cell lines differed in their relative expression of tenascin in the cell-associated and supernatant compartments. Glioma cell line tenascin production was not uniformly stimulated by changes in fetal bovine serum concentration in the growth media. In most glioma cell lines and normal tissue extracts, reducing Western blots and Northern blots revealed two tenascin species, respectively: a major 340 kDa polypeptide and a 9 kb RNA transcript accompanied by a less intense 250 kDa polypeptide and 7 kDa RNA species. In U-87 MG and in normal adult kidney extracts, however, the 250 kDa band and 7 kb transcript were more prominent. Quantitation of tenascin in the glioma lines revealed variable levels that were significantly higher than those in the tissue extracts.

Duke Scholars

Darell Doty Bigner
Author Darell Doty Bigner Neurosurgery
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Published In

J Neuroimmunol

DOI

10.1016/0165-5728(92)90029-k

ISSN

0165-5728

Publication Date

January 1992

Volume

36

Issue

1

Start / End Page

41 / 55

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Tissue Extracts
  • Tenascin
  • Reference Values
  • RNA, Messenger
  • Neurology & Neurosurgery
  • Kidney
  • Immunoblotting
  • Humans
  • Glioma
 

Citation

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Ventimiglia, J. B., Wikstrand, C. J., Ostrowski, L. E., Bourdon, M. A., Lightner, V. A., & Bigner, D. D. (1992). Tenascin expression in human glioma cell lines and normal tissues. J Neuroimmunol, 36(1), 41–55. https://doi.org/10.1016/0165-5728(92)90029-k
Ventimiglia, J. B., C. J. Wikstrand, L. E. Ostrowski, M. A. Bourdon, V. A. Lightner, and D. D. Bigner. “Tenascin expression in human glioma cell lines and normal tissues.” J Neuroimmunol 36, no. 1 (January 1992): 41–55. https://doi.org/10.1016/0165-5728(92)90029-k.
Ventimiglia JB, Wikstrand CJ, Ostrowski LE, Bourdon MA, Lightner VA, Bigner DD. Tenascin expression in human glioma cell lines and normal tissues. J Neuroimmunol. 1992 Jan;36(1):41–55.
Ventimiglia, J. B., et al. “Tenascin expression in human glioma cell lines and normal tissues.” J Neuroimmunol, vol. 36, no. 1, Jan. 1992, pp. 41–55. Pubmed, doi:10.1016/0165-5728(92)90029-k.
Ventimiglia JB, Wikstrand CJ, Ostrowski LE, Bourdon MA, Lightner VA, Bigner DD. Tenascin expression in human glioma cell lines and normal tissues. J Neuroimmunol. 1992 Jan;36(1):41–55.
Journal cover image

Published In

J Neuroimmunol

DOI

10.1016/0165-5728(92)90029-k

ISSN

0165-5728

Publication Date

January 1992

Volume

36

Issue

1

Start / End Page

41 / 55

Location

Netherlands

Related Subject Headings

  • Tumor Cells, Cultured
  • Tissue Extracts
  • Tenascin
  • Reference Values
  • RNA, Messenger
  • Neurology & Neurosurgery
  • Kidney
  • Immunoblotting
  • Humans
  • Glioma