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Meiotic recombination involving heterozygous large insertions in Saccharomyces cerevisiae: formation and repair of large, unpaired DNA loops.

Publication ,  Journal Article
Kearney, HM; Kirkpatrick, DT; Gerton, JL; Petes, TD
Published in: Genetics
August 2001

Meiotic recombination in Saccharomyces cerevisiae involves the formation of heteroduplexes, duplexes containing DNA strands derived from two different homologues. If the two strands of DNA differ by an insertion or deletion, the heteroduplex will contain an unpaired DNA loop. We found that unpaired loops as large as 5.6 kb can be accommodated within a heteroduplex. Repair of these loops involved the nucleotide excision repair (NER) enzymes Rad1p and Rad10p and the mismatch repair (MMR) proteins Msh2p and Msh3p, but not several other NER (Rad2p and Rad14p) and MMR (Msh4p, Msh6p, Mlh1p, Pms1p, Mlh2p, Mlh3p) proteins. Heteroduplexes were also formed with DNA strands derived from alleles containing two different large insertions, creating a large "bubble"; repair of this substrate was dependent on Rad1p. Although meiotic recombination events in yeast are initiated by double-strand DNA breaks (DSBs), we showed that DSBs occurring within heterozygous insertions do not stimulate interhomologue recombination.

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Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

August 2001

Volume

158

Issue

4

Start / End Page

1457 / 1476

Location

United States

Related Subject Headings

  • Single-Strand Specific DNA and RNA Endonucleases
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Nucleic Acid Heteroduplexes
  • Nucleic Acid Conformation
  • MutS Homolog 3 Protein
  • MutS Homolog 2 Protein
  • Models, Genetic
  • Meiosis
 

Citation

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Kearney, H. M., Kirkpatrick, D. T., Gerton, J. L., & Petes, T. D. (2001). Meiotic recombination involving heterozygous large insertions in Saccharomyces cerevisiae: formation and repair of large, unpaired DNA loops. Genetics, 158(4), 1457–1476. https://doi.org/10.1093/genetics/158.4.1457
Kearney, H. M., D. T. Kirkpatrick, J. L. Gerton, and T. D. Petes. “Meiotic recombination involving heterozygous large insertions in Saccharomyces cerevisiae: formation and repair of large, unpaired DNA loops.Genetics 158, no. 4 (August 2001): 1457–76. https://doi.org/10.1093/genetics/158.4.1457.
Kearney, H. M., et al. “Meiotic recombination involving heterozygous large insertions in Saccharomyces cerevisiae: formation and repair of large, unpaired DNA loops.Genetics, vol. 158, no. 4, Aug. 2001, pp. 1457–76. Pubmed, doi:10.1093/genetics/158.4.1457.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

August 2001

Volume

158

Issue

4

Start / End Page

1457 / 1476

Location

United States

Related Subject Headings

  • Single-Strand Specific DNA and RNA Endonucleases
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Nucleic Acid Heteroduplexes
  • Nucleic Acid Conformation
  • MutS Homolog 3 Protein
  • MutS Homolog 2 Protein
  • Models, Genetic
  • Meiosis