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Linker for activation of T cells (LAT), a novel immunohistochemical marker for T cells, NK cells, mast cells, and megakaryocytes: evaluation in normal and pathological conditions.

Publication ,  Journal Article
Facchetti, F; Chan, JK; Zhang, W; Tironi, A; Chilosi, M; Parolini, S; Notarangelo, LD; Samelson, LE
Published in: Am J Pathol
April 1999

LAT (linker for activation of T cells) is an integral membrane protein of 36-38 kd that plays an important role in T cell activation. Using a rabbit polyclonal antibody generated against the cytosolic portion of LAT, we investigated the immunohistochemical expression of LAT in normal and pathological hematolymphoid tissues. LAT reacts with human T cells in paraffin sections, including decalcified bone marrow trephines. LAT appears early in T cells at the thymocyte stage and before TdT expression in embryos, and is expressed in peripheral lymphoid tissues, without restriction to any T cell subpopulations. In addition to T cells, natural killer (NK) cells (evaluated with flow cytometry), megakaryocytes and mast cells are also LAT-positive, whereas B cells and other myeloid and monocytic derived cells are negative. Tested on a total of 264 paraffin-embedded tissue biopsies, LAT reacted with the great majority (96.8%) of T/NK-cell neoplasms, covering the full range of T cell maturation. Although antibodies to both LAT and CD3 had a similarly high sensitivity in the staining of T/NK-cell lymphomas, when used in conjunction, they successfully identified a higher number of cases (98.4%). Atypical megakaryocytes from different hematological disorders, as well as mast cells in mastocytosis were also LAT-positive, but all neoplasms of B cell origin, Hodgkin's lymphomas, and several nonlymphoid malignancies were negative. These data indicate that the anti-LAT antibody may be of value to diagnostic histopathologists for the identification of T cell neoplasms.

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Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

April 1999

Volume

154

Issue

4

Start / End Page

1037 / 1046

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Spleen
  • Phosphoproteins
  • Pathology
  • Membrane Proteins
  • Megakaryocytes
  • Mast Cells
  • Lymphoma
  • Killer Cells, Natural
 

Citation

APA
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MLA
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Facchetti, F., Chan, J. K., Zhang, W., Tironi, A., Chilosi, M., Parolini, S., … Samelson, L. E. (1999). Linker for activation of T cells (LAT), a novel immunohistochemical marker for T cells, NK cells, mast cells, and megakaryocytes: evaluation in normal and pathological conditions. Am J Pathol, 154(4), 1037–1046. https://doi.org/10.1016/S0002-9440(10)65356-4
Facchetti, F., J. K. Chan, W. Zhang, A. Tironi, M. Chilosi, S. Parolini, L. D. Notarangelo, and L. E. Samelson. “Linker for activation of T cells (LAT), a novel immunohistochemical marker for T cells, NK cells, mast cells, and megakaryocytes: evaluation in normal and pathological conditions.Am J Pathol 154, no. 4 (April 1999): 1037–46. https://doi.org/10.1016/S0002-9440(10)65356-4.
Facchetti, F., et al. “Linker for activation of T cells (LAT), a novel immunohistochemical marker for T cells, NK cells, mast cells, and megakaryocytes: evaluation in normal and pathological conditions.Am J Pathol, vol. 154, no. 4, Apr. 1999, pp. 1037–46. Pubmed, doi:10.1016/S0002-9440(10)65356-4.
Facchetti F, Chan JK, Zhang W, Tironi A, Chilosi M, Parolini S, Notarangelo LD, Samelson LE. Linker for activation of T cells (LAT), a novel immunohistochemical marker for T cells, NK cells, mast cells, and megakaryocytes: evaluation in normal and pathological conditions. Am J Pathol. 1999 Apr;154(4):1037–1046.
Journal cover image

Published In

Am J Pathol

DOI

ISSN

0002-9440

Publication Date

April 1999

Volume

154

Issue

4

Start / End Page

1037 / 1046

Location

United States

Related Subject Headings

  • Thymus Gland
  • T-Lymphocytes
  • Spleen
  • Phosphoproteins
  • Pathology
  • Membrane Proteins
  • Megakaryocytes
  • Mast Cells
  • Lymphoma
  • Killer Cells, Natural