Regulation of ZAP-70 intracellular localization: visualization with the green fluorescent protein.
To investigate the cellular dynamics of ZAP-70, we have studied the distribution and regulation of its intracellular location using a ZAP-70 green fluorescent protein chimera. Initial experiments in epithelial cells indicated that ZAP-70 is diffusely located throughout the quiescent cell, and accumulates at the plasma membrane upon cellular activation, a phenotype enhanced by the coexpression of Lck and the initiation of ZAP-70 kinase activity. Subsequent studies in T cells confirmed this phenotype. Intriguingly, a large amount of ZAP-70, both chimeric and endogenous, resides in the nucleus of quiescent and activated cells. Nuclear ZAP-70 becomes tyrosine phosphorylated upon stimulation via the T cell receptor, indicating that it may have an important biologic function.
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- ZAP-70 Protein-Tyrosine Kinase
- Tyrosine
- Staining and Labeling
- Recombinant Fusion Proteins
- Receptors, Antigen, T-Cell
- Protein-Tyrosine Kinases
- Phosphorylation
- Nuclear Envelope
- Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
- Luminescent Proteins
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- ZAP-70 Protein-Tyrosine Kinase
- Tyrosine
- Staining and Labeling
- Recombinant Fusion Proteins
- Receptors, Antigen, T-Cell
- Protein-Tyrosine Kinases
- Phosphorylation
- Nuclear Envelope
- Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
- Luminescent Proteins