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Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: a soluble, secreted CD30 homologue.

Publication ,  Journal Article
Panus, JF; Smith, CA; Ray, CA; Smith, TD; Patel, DD; Pickup, DJ
Published in: Proc Natl Acad Sci U S A
June 11, 2002
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Cowpox virus (Brighton Red strain) possesses one of the largest genomes in the Orthopoxvirus genus. Sequence analysis of a region of the genome that is type-specific for cowpox virus identified a gene, vCD30, encoding a soluble, secreted protein that is the fifth member of the tumor necrosis factor receptor family known to be encoded by cowpox virus. The vCD30 protein contains 110 aa, including a 21-residue signal peptide, a potential O-linked glycosylation site, and a 58-aa sequence sharing 51-59% identity with highly conserved extracellular segments of both mouse and human CD30. A vCD30Fc fusion protein binds CD153 (CD30 ligand) specifically, and it completely inhibits CD153/CD30 interactions. Although the functions of CD30 are not well understood, the existence of vCD30 suggests that the cellular receptor plays a significant role in normal immune responses. Viral inhibition of CD30 also lends support to the potential therapeutic value of targeting CD30 in human inflammatory and autoimmune diseases.

Duke Scholars

Dhavalkumar Dhirajlal Patel
Author Dhavalkumar Dhirajlal Patel Medicine, Rheumatology and Immunology
David James Pickup
Author David James Pickup Molecular Genetics and Microbiology
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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.122238599

ISSN

0027-8424

Publication Date

June 11, 2002

Volume

99

Issue

12

Start / End Page

8348 / 8353

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Recombination, Genetic
  • Recombinant Fusion Proteins
  • Receptors, Tumor Necrosis Factor
  • Plasmids
  • Osteosarcoma
  • Molecular Sequence Data
 

Citation

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Panus, J. F., Smith, C. A., Ray, C. A., Smith, T. D., Patel, D. D., & Pickup, D. J. (2002). Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: a soluble, secreted CD30 homologue. Proc Natl Acad Sci U S A, 99(12), 8348–8353. https://doi.org/10.1073/pnas.122238599
Panus, Joanne Fanelli, Craig A. Smith, Caroline A. Ray, Terri Davis Smith, Dhavalkumar D. Patel, and David J. Pickup. “Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: a soluble, secreted CD30 homologue.” Proc Natl Acad Sci U S A 99, no. 12 (June 11, 2002): 8348–53. https://doi.org/10.1073/pnas.122238599.
Panus JF, Smith CA, Ray CA, Smith TD, Patel DD, Pickup DJ. Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: a soluble, secreted CD30 homologue. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8348–53.
Panus, Joanne Fanelli, et al. “Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: a soluble, secreted CD30 homologue.” Proc Natl Acad Sci U S A, vol. 99, no. 12, June 2002, pp. 8348–53. Pubmed, doi:10.1073/pnas.122238599.
Panus JF, Smith CA, Ray CA, Smith TD, Patel DD, Pickup DJ. Cowpox virus encodes a fifth member of the tumor necrosis factor receptor family: a soluble, secreted CD30 homologue. Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8348–8353.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.122238599

ISSN

0027-8424

Publication Date

June 11, 2002

Volume

99

Issue

12

Start / End Page

8348 / 8353

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Recombination, Genetic
  • Recombinant Fusion Proteins
  • Receptors, Tumor Necrosis Factor
  • Plasmids
  • Osteosarcoma
  • Molecular Sequence Data