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Cowpox virus genome encodes a second soluble homologue of cellular TNF receptors, distinct from CrmB, that binds TNF but not LT alpha.

Publication ,  Journal Article
Smith, CA; Hu, FQ; Smith, TD; Richards, CL; Smolak, P; Goodwin, RG; Pickup, DJ
Published in: Virology
September 1, 1996

We show the cowpox genome (Brighton Red strain) contains a single copy gene, crmC, expressed at late times during viral infection, encoding a soluble, secreted protein whose sequence marks it as a new member of the TNF receptor family. The cysteine-rich protein contains 186 amino acids, the N-terminal 21 of which constitute a signal peptide, and two potential N-linked glycosylation sites. The approximately 25-kDa recombinant protein binds TNF specifically and completely inhibits TNF-mediated cytolysis. The strongest sequence homologues are the ligand-binding regions of the type II cellular TNF receptor (TNFRII) and CrmB, a distinct pox virus gene also encoding a soluble TNF binding protein. Unlike TNFRII and CrmB, CrmC does not bind lymphotoxin (LT alpha, TNF beta) and lacks the conserved (but nonhomologous) approximately 150-residue C-terminal domain of CrmB proteins. The presumed function of CrmC is viral inhibition of host-elicited TNF.

Duke Scholars

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

September 1, 1996

Volume

223

Issue

1

Start / End Page

132 / 147

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Proteins
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Solubility
  • Sequence Homology, Amino Acid
  • Receptors, Tumor Necrosis Factor
  • Molecular Sequence Data
  • Mice
 

Citation

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Smith, C. A., Hu, F. Q., Smith, T. D., Richards, C. L., Smolak, P., Goodwin, R. G., & Pickup, D. J. (1996). Cowpox virus genome encodes a second soluble homologue of cellular TNF receptors, distinct from CrmB, that binds TNF but not LT alpha. Virology, 223(1), 132–147. https://doi.org/10.1006/viro.1996.0462
Smith, C. A., F. Q. Hu, T. D. Smith, C. L. Richards, P. Smolak, R. G. Goodwin, and D. J. Pickup. “Cowpox virus genome encodes a second soluble homologue of cellular TNF receptors, distinct from CrmB, that binds TNF but not LT alpha.Virology 223, no. 1 (September 1, 1996): 132–47. https://doi.org/10.1006/viro.1996.0462.
Smith CA, Hu FQ, Smith TD, Richards CL, Smolak P, Goodwin RG, et al. Cowpox virus genome encodes a second soluble homologue of cellular TNF receptors, distinct from CrmB, that binds TNF but not LT alpha. Virology. 1996 Sep 1;223(1):132–47.
Smith, C. A., et al. “Cowpox virus genome encodes a second soluble homologue of cellular TNF receptors, distinct from CrmB, that binds TNF but not LT alpha.Virology, vol. 223, no. 1, Sept. 1996, pp. 132–47. Pubmed, doi:10.1006/viro.1996.0462.
Smith CA, Hu FQ, Smith TD, Richards CL, Smolak P, Goodwin RG, Pickup DJ. Cowpox virus genome encodes a second soluble homologue of cellular TNF receptors, distinct from CrmB, that binds TNF but not LT alpha. Virology. 1996 Sep 1;223(1):132–147.
Journal cover image

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

September 1, 1996

Volume

223

Issue

1

Start / End Page

132 / 147

Location

United States

Related Subject Headings

  • Virus Replication
  • Virology
  • Viral Proteins
  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Solubility
  • Sequence Homology, Amino Acid
  • Receptors, Tumor Necrosis Factor
  • Molecular Sequence Data
  • Mice