Skip to main content
Journal cover image

The 120-kDa soluble ectodomain of type XVII collagen is recognized by autoantibodies in patients with pemphigoid and linear IgA dermatosis.

Publication ,  Journal Article
Roh, JY; Yee, C; Lazarova, Z; Hall, RP; Yancey, KB
Published in: Br J Dermatol
July 2000

BACKGROUND: Type XVII collagen promotes adhesion of basal keratinocytes to epidermal basement membrane, and is the target of disease in patients with certain inherited or acquired blistering diseases. Two forms of type XVII collagen are produced by cultured human keratinocytes: a 180-kDa full-length, transmembrane protein, and a recently identified 120-kDa soluble fragment that corresponds to its collagenous ectodomain. OBJECTIVES: We aimed to determine the incidence and pattern of reactivity of autoantibodies against the 180- and 120-kDa forms of type XVII collagen in sera from 40 patients with bullous pemphigoid (BP), pemphigoid gestationis or cicatricial pemphigoid (CP), as well as six patients with linear IgA dermatosis (LAD). METHODS: Various immunochemical techniques were used. RESULTS: These studies found that the 120-kDa fragment of type XVII collagen was bound by circulating autoantibodies in 13 of 38 patients with BP or CP and all six patients with LAD. While many pemphigoid sera had specific reactivity against one but not both forms of this protein, autoantibodies from patients with LAD bound only the soluble ectodomain. CONCLUSIONS: These findings are consistent with the presence of both neoepitopes and cross-reactive epitopes on the ectodomain of type XVII collagen. The finding that sera from patients with LAD showed specific reactivity to epidermal basement membrane suggests that such neoepitopes are present in human skin and that their targeting by autoantibodies may contribute to disease pathogenesis.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Br J Dermatol

DOI

ISSN

0007-0963

Publication Date

July 2000

Volume

143

Issue

1

Start / End Page

104 / 111

Location

England

Related Subject Headings

  • Protein Binding
  • Pemphigoid, Bullous
  • Pemphigoid, Benign Mucous Membrane
  • Non-Fibrillar Collagens
  • Nerve Tissue Proteins
  • Middle Aged
  • Male
  • Keratinocytes
  • Immunoglobulin G
  • Immunoglobulin A
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Roh, J. Y., Yee, C., Lazarova, Z., Hall, R. P., & Yancey, K. B. (2000). The 120-kDa soluble ectodomain of type XVII collagen is recognized by autoantibodies in patients with pemphigoid and linear IgA dermatosis. Br J Dermatol, 143(1), 104–111. https://doi.org/10.1046/j.1365-2133.2000.03598.x
Roh, J. Y., C. Yee, Z. Lazarova, R. P. Hall, and K. B. Yancey. “The 120-kDa soluble ectodomain of type XVII collagen is recognized by autoantibodies in patients with pemphigoid and linear IgA dermatosis.Br J Dermatol 143, no. 1 (July 2000): 104–11. https://doi.org/10.1046/j.1365-2133.2000.03598.x.
Roh, J. Y., et al. “The 120-kDa soluble ectodomain of type XVII collagen is recognized by autoantibodies in patients with pemphigoid and linear IgA dermatosis.Br J Dermatol, vol. 143, no. 1, July 2000, pp. 104–11. Pubmed, doi:10.1046/j.1365-2133.2000.03598.x.
Journal cover image

Published In

Br J Dermatol

DOI

ISSN

0007-0963

Publication Date

July 2000

Volume

143

Issue

1

Start / End Page

104 / 111

Location

England

Related Subject Headings

  • Protein Binding
  • Pemphigoid, Bullous
  • Pemphigoid, Benign Mucous Membrane
  • Non-Fibrillar Collagens
  • Nerve Tissue Proteins
  • Middle Aged
  • Male
  • Keratinocytes
  • Immunoglobulin G
  • Immunoglobulin A