Skip to main content
Journal cover image

Laser-induced differential fluorescence for cancer diagnosis without biopsy

Publication ,  Journal Article
Vo-Dinh, T; Panjehpour, M; Overholt, BF; Buckley, P
Published in: Applied Spectroscopy
January 1, 1997

An optical diagnostic procedure based on laser-induced fluorescence was developed for direct in vivo cancer diagnosis without requiring biopsy. The methodology was applied in a clinical study involving over 100 patients in order to differentiate normal tissue from malignant tumors of the esophagus. Endogenous fluorescence of normal and malignant tissues was measured directly with the use of a fiber-optic probe inserted through an endoscope. The measurements were performed in vivo during routine endoscopy. Detection of the fluorescence signal from the tissue was performed with the use of laser excitation. This report describes the differential normalized fluorescence (DNF) procedure using the amplified spectral differences between the normalized fluorescence of malignant tissue and normal mucosa. The results of this DNF approach were compared with histopathology results of the biopsy samples and indicated excellent agreement in the classification of normal tissue and malignant tumors for the samples investigated. Data related to various grades of Barrett's esophagus are discussed. The DNF procedure could lead to the development of a rapid and cost-effective technique for cancer diagnosis.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Applied Spectroscopy

DOI

ISSN

0003-7028

Publication Date

January 1, 1997

Volume

51

Issue

1

Start / End Page

58 / 63

Related Subject Headings

  • Analytical Chemistry
  • 0913 Mechanical Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0301 Analytical Chemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Vo-Dinh, T., Panjehpour, M., Overholt, B. F., & Buckley, P. (1997). Laser-induced differential fluorescence for cancer diagnosis without biopsy. Applied Spectroscopy, 51(1), 58–63. https://doi.org/10.1366/0003702971938768
Vo-Dinh, T., M. Panjehpour, B. F. Overholt, and P. Buckley. “Laser-induced differential fluorescence for cancer diagnosis without biopsy.” Applied Spectroscopy 51, no. 1 (January 1, 1997): 58–63. https://doi.org/10.1366/0003702971938768.
Vo-Dinh T, Panjehpour M, Overholt BF, Buckley P. Laser-induced differential fluorescence for cancer diagnosis without biopsy. Applied Spectroscopy. 1997 Jan 1;51(1):58–63.
Vo-Dinh, T., et al. “Laser-induced differential fluorescence for cancer diagnosis without biopsy.” Applied Spectroscopy, vol. 51, no. 1, Jan. 1997, pp. 58–63. Scopus, doi:10.1366/0003702971938768.
Vo-Dinh T, Panjehpour M, Overholt BF, Buckley P. Laser-induced differential fluorescence for cancer diagnosis without biopsy. Applied Spectroscopy. 1997 Jan 1;51(1):58–63.
Journal cover image

Published In

Applied Spectroscopy

DOI

ISSN

0003-7028

Publication Date

January 1, 1997

Volume

51

Issue

1

Start / End Page

58 / 63

Related Subject Headings

  • Analytical Chemistry
  • 0913 Mechanical Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0301 Analytical Chemistry