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Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE)

Publication ,  Journal Article
Cheng, YF; Piccard, RD; Vo-Dinh, T
Published in: Applied Spectroscopy
January 1, 1990

This paper describes a new instrument system combining capillary-zone electrophoresis (CZE) separation and charge-coupled device (CCD) fluorescence detection. The performance of this CCD-CZE system is evaluated and compared with the performance of a photomultiplier tube (PMT) detector, with the use of signal-to-noise (S/N) ratio and acquisition time as measures of performance. The feasibility of on-column fluorescence spectrometric detection is demonstrated, and a three-dimensional electrophorogram is presented to show the spectral and time resolution obtained by injection of 2 femtomoles (5 nanoliters of 4.0 × 10-7 M) of fluorescein into the capillary column. The software us a 'peak sum' method to achieve optimum S/N ration.

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Published In

Applied Spectroscopy

DOI

ISSN

0003-7028

Publication Date

January 1, 1990

Volume

44

Issue

5

Start / End Page

755 / 765

Related Subject Headings

  • Analytical Chemistry
  • 0913 Mechanical Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0301 Analytical Chemistry
 

Citation

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Cheng, Y. F., Piccard, R. D., & Vo-Dinh, T. (1990). Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE). Applied Spectroscopy, 44(5), 755–765. https://doi.org/10.1366/0003702904087082
Cheng, Y. F., R. D. Piccard, and T. Vo-Dinh. “Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE).” Applied Spectroscopy 44, no. 5 (January 1, 1990): 755–65. https://doi.org/10.1366/0003702904087082.
Cheng YF, Piccard RD, Vo-Dinh T. Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE). Applied Spectroscopy. 1990 Jan 1;44(5):755–65.
Cheng, Y. F., et al. “Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE).” Applied Spectroscopy, vol. 44, no. 5, Jan. 1990, pp. 755–65. Scopus, doi:10.1366/0003702904087082.
Cheng YF, Piccard RD, Vo-Dinh T. Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE). Applied Spectroscopy. 1990 Jan 1;44(5):755–765.
Journal cover image

Published In

Applied Spectroscopy

DOI

ISSN

0003-7028

Publication Date

January 1, 1990

Volume

44

Issue

5

Start / End Page

755 / 765

Related Subject Headings

  • Analytical Chemistry
  • 0913 Mechanical Engineering
  • 0306 Physical Chemistry (incl. Structural)
  • 0301 Analytical Chemistry