Charge-coupled device fluorescence detection for capillary-zone electrophoresis (CCD-CZE)

This paper describes a new instrument system combining capillary-zone electrophoresis (CZE) separation and charge-coupled device (CCD) fluorescence detection. The performance of this CCD-CZE system is evaluated and compared with the performance of a photomultiplier tube (PMT) detector, with the use of signal-to-noise (S/N) ratio and acquisition time as measures of performance. The feasibility of on-column fluorescence spectrometric detection is demonstrated, and a three-dimensional electrophorogram is presented to show the spectral and time resolution obtained by injection of 2 femtomoles (5 nanoliters of 4.0 × 10-7 M) of fluorescein into the capillary column. The software us a 'peak sum' method to achieve optimum S/N ration.

Duke Scholars

Author Tuan Vo-Dinh Biomedical Engineering