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Regulation of I-309 gene expression in human monocytes by endogenous interleukin-1.

Publication ,  Journal Article
Selvan, RS; Zhou, LJ; Krangel, MS
Published in: Eur J Immunol
March 1997

Activated human monocytes are a source of numerous beta-chemokines. The present study was conducted to determine whether these cells produce the human beta-chemokine I-309 and to compare the induction requirements of I-309 to those of other beta-chemokines. We demonstrate that appropriately stimulated adherence-purified human peripheral blood monocytes express I-309 transcripts and secreted I-309 protein. Two stimuli, immobilized IgG and lipopolysaccharide (LPS), synergize strongly to induce I-309 gene expression. We further demonstrate that the production of endogenous interleukin (IL)-1alpha plays a crucial role in I-309 induction. Thus, neutralization of endogenous IL-1alpha using an anti-IL-1alpha antiserum inhibits the induction of I-309 transcripts in response to stimulation with immobilized IgG and LPS, and exogenous IL-1alpha or IL-1beta induces I-309 transcripts in monocytes stimulated with immobilized IgG. Immobilized IgG and LPS have the opposite effect on monocyte chemoattractant protein-1 (MCP-1) gene expression, in that the induction observed with either stimulus alone is diminished using the two stimuli in combination. Furthermore, endogenous and exogenous IL-1 can be either stimulatory or inhibitory for MCP-1 gene expression depending on other signals delivered to the monocytes. Immobilized IgG and LPS synergize to induce macrophage inflammatory protein-1alpha transcripts, but endogenous IL-1 does not play a significant role. Thus, each of these beta-chemokine genes is under distinct regulatory control in human monocytes.

Duke Scholars

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

March 1997

Volume

27

Issue

3

Start / End Page

687 / 694

Location

Germany

Related Subject Headings

  • Transcription, Genetic
  • RNA, Messenger
  • Monocytes
  • Macrophage Inflammatory Proteins
  • Lipopolysaccharides
  • Interleukin-1
  • Immunology
  • Immunoglobulin G
  • Humans
  • Gene Expression
 

Citation

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Selvan, R. S., Zhou, L. J., & Krangel, M. S. (1997). Regulation of I-309 gene expression in human monocytes by endogenous interleukin-1. Eur J Immunol, 27(3), 687–694. https://doi.org/10.1002/eji.1830270317
Selvan, R. S., L. J. Zhou, and M. S. Krangel. “Regulation of I-309 gene expression in human monocytes by endogenous interleukin-1.Eur J Immunol 27, no. 3 (March 1997): 687–94. https://doi.org/10.1002/eji.1830270317.
Selvan RS, Zhou LJ, Krangel MS. Regulation of I-309 gene expression in human monocytes by endogenous interleukin-1. Eur J Immunol. 1997 Mar;27(3):687–94.
Selvan, R. S., et al. “Regulation of I-309 gene expression in human monocytes by endogenous interleukin-1.Eur J Immunol, vol. 27, no. 3, Mar. 1997, pp. 687–94. Pubmed, doi:10.1002/eji.1830270317.
Selvan RS, Zhou LJ, Krangel MS. Regulation of I-309 gene expression in human monocytes by endogenous interleukin-1. Eur J Immunol. 1997 Mar;27(3):687–694.
Journal cover image

Published In

Eur J Immunol

DOI

ISSN

0014-2980

Publication Date

March 1997

Volume

27

Issue

3

Start / End Page

687 / 694

Location

Germany

Related Subject Headings

  • Transcription, Genetic
  • RNA, Messenger
  • Monocytes
  • Macrophage Inflammatory Proteins
  • Lipopolysaccharides
  • Interleukin-1
  • Immunology
  • Immunoglobulin G
  • Humans
  • Gene Expression